Site-Specific Cleavage of Single-Stranded DNA by a Hemophilus Restriction Endonuclease

Single-stranded viral DNA of bacteriophage f1 is cleaved into specific fragments by endo R· HaeIII, a restriction endonuclease isolated from Hemophilus aegyptius. The sites of the single strand cleavage correspond to those of the double strand cleavage. A single-stranded DNA fragment containing only...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1975-07, Vol.72 (7), p.2555-2558
Main Authors: Horiuchi, Kensuke, Zinder, Norton D.
Format: Article
Language:English
Subjects:
Bacteriophages
Base Sequence
Biochemistry
Chromosome Mapping
Coliphages
DNA
DNA Restriction Enzymes - metabolism
DNA Viruses
DNA, Single-Stranded
DNA, Viral
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Endonucleases
Enzymes
Gels
Haemophilus
Haemophilus - enzymology
Nucleic Acid Denaturation
Nucleic Acid Renaturation
Nucleotide sequences
Single stranded DNA
Viral DNA
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Description
Summary:Single-stranded viral DNA of bacteriophage f1 is cleaved into specific fragments by endo R· HaeIII, a restriction endonuclease isolated from Hemophilus aegyptius. The sites of the single strand cleavage correspond to those of the double strand cleavage. A single-stranded DNA fragment containing only one HaeIII site is also cleaved by this enzyme. This observation suggests that the reaction of single-stranded DNA cleavage does not require the formation of a symmetrical double-stranded structure that would result from the intramolecular base-pairing between two different HaeIII sites. Other restriction endonucleases may also cleave single-stranded DNA.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.72.7.2555