Surface Ruffles as Markers for Studies of Cell Transformation by Rous Sarcoma Virus

Confluent chick embryo fibroblasts infected with the Ts68 mutant of Rous sarcoma virus were examined by scanning electron microscopy at the permissive (36 degrees) and nonpermissive (41 degrees) temperatures for transformation. Infected cells shifted from 41 degrees to 36 degrees undergo a change in...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1975-08, Vol.72 (8), p.3144-3148
Main Authors: Ambros, Victor R., Chen, Lan Bo, Buchanan, John M.
Format: Article
Language:English
Subjects:
Animals
Avian Sarcoma Viruses - metabolism
Cell Transformation, Neoplastic - drug effects
Cells
Chick Embryo
Cultured cells
Cycloheximide - pharmacology
Delta cells
Embryos
Fibroblasts
Fibroblasts - metabolism
Fibroblasts - ultrastructure
Microscopy, Electron, Scanning
Protein synthesis
Rous sarcoma virus
Ruffles
Temperature
Viral morphology
Viruses
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Description
Summary:Confluent chick embryo fibroblasts infected with the Ts68 mutant of Rous sarcoma virus were examined by scanning electron microscopy at the permissive (36 degrees) and nonpermissive (41 degrees) temperatures for transformation. Infected cells shifted from 41 degrees to 36 degrees undergo a change in shape from elongated to rounded. This process is preceded by the appearance of surface ruffles on the cell. These surface ruffles are not observed on cells maintained at 41 degrees, appear as early as 0.5 hr after a shift to 36 degrees, and are common on cells maintained at 36 degrees. By 3.5 hr after the shift from 41 degrees to 36 degrees, cultures appear fully transformed by the criteria of cell roundedness and the presence of surface ruffles. This surface alteration of cells is the earliest event of those so far reported during the transformation process and is not dependent upon protein synthesis and extracellular plasminogen during the period of temperature shift.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.72.8.3144