Isolation and Characterization of Polyoma Virus Mutants able to Develop in Embryonal Carcinoma Cells

Embryonal carcinoma (EC) mouse cells have been shown to be resistant to infection by retroviruses and small oncogenic DNA viruses, including simian virus 40 and polyoma. When allowed to differentiate, in vitro or in vivo, EC cells become as susceptible to these viruses as differentiated mouse cell l...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1980-02, Vol.77 (2), p.1068-1072
Main Authors: Vasseur, Marc, Kress, Chantal, Montreau, Nicole, Blangy, Daniel
Format: Article
Language:English
Subjects:
Base Sequence
Cell Line
Cell lines
Cellular differentiation
DNA Restriction Enzymes
DNA, Viral - genetics
Embryonal carcinoma stem cells
Embryonic stem cells
Genomes
Infections
Multipotent stem cells
Mutation
Neoplasms, Experimental - microbiology
Polyomavirus - genetics
Stem cells
Teratoma - microbiology
Viral tumor antigens
Virus Replication
Viruses
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Summary:Embryonal carcinoma (EC) mouse cells have been shown to be resistant to infection by retroviruses and small oncogenic DNA viruses, including simian virus 40 and polyoma. When allowed to differentiate, in vitro or in vivo, EC cells become as susceptible to these viruses as differentiated mouse cell lines are. In order to study the relationships between differentiation of EC cells and viral expression, we have isolated and characterized several polyoma mutants that can express early and late functions in undifferentiated EC cells. These mutants, which arose spontaneously during high-multiplicity infection of PCD3 cells (a differentiated fibroblast-like cell line derived from PCC3 EC cells), were selected on PCC4 cells (undifferentiated EC cells) and twice plaque purified. Restriction enzyme analysis of the DNA from several mutants has shown that they all exhibit an additional sequence located in the Pvu II endonuclease fragment 4, close to the junction between Hpa II endonuclease fragments 3 and 5. The size of the insertion varies from 10 to 50 base pairs. The biological properties, including oncogenicity, transforming ability, host range, and burst size of the mutants so far analyzed are similar to those of wild-type virus.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.2.1068