Leukotriene D: A Slow Reacting Substance from Rat Basophilic Leukemia Cells

A slow reacting substance produced by rat basophilic leukemia cells, treated with ionophore A23187, was characterized by spectroscopic methods, enzymatic conversions, and chemical degradations as 5-hydroxy-6-S-cysteinylglycyl-7,9,11,14-eicosatetraenoic acid (leukotriene D). γ -Glutamyltranspeptidase...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1980-04, Vol.77 (4), p.2014-2017
Main Authors: Orning, Lars, Hammarstrom, Sven, Samuelsson, Bengt
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - analysis
Animals
Arachidonic Acids - analysis
Autacoids - analysis
Basophils - analysis
Bioassay
Enzymes
Ethanol
Ileum
Leukemia
Leukemia - analysis
Leukotrienes
Lipoxygenase - metabolism
Rats
Sequence analysis
Soybeans
Spectrophotometry, Ultraviolet
SRS-A
Sulfur
Ultraviolet spectrum
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cited_by cdi_FETCH-LOGICAL-c455t-75ab4843517c49f229a7ff824371966ca73c6ee00f84f82bb6de7c6af0cd03893
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container_end_page 2017
container_issue 4
container_start_page 2014
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 77
creator Orning, Lars
Hammarstrom, Sven
Samuelsson, Bengt
description A slow reacting substance produced by rat basophilic leukemia cells, treated with ionophore A23187, was characterized by spectroscopic methods, enzymatic conversions, and chemical degradations as 5-hydroxy-6-S-cysteinylglycyl-7,9,11,14-eicosatetraenoic acid (leukotriene D). γ -Glutamyltranspeptidase [γ -glutamyltransferase; (5-glutamyl)-peptide: amino-acid 5-glutamyltransferase, EC 2.3.2.2] converted leukotriene C to a product identical to leukotriene D. This suggests that the stereochemistry of the arachidonyl moiety of leukotrienes C and D is the same [5(S)-6(R)-7,9-trans-11,14-cis]. Leukotriene D induces a faster contraction and, on a molar basis, is more potent than leukotriene C in the isolated guinea pig ileum bioassay.
doi_str_mv 10.1073/pnas.77.4.2014
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This suggests that the stereochemistry of the arachidonyl moiety of leukotrienes C and D is the same [5(S)-6(R)-7,9-trans-11,14-cis]. Leukotriene D induces a faster contraction and, on a molar basis, is more potent than leukotriene C in the isolated guinea pig ileum bioassay.</description><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Arachidonic Acids - analysis</subject><subject>Autacoids - analysis</subject><subject>Basophils - analysis</subject><subject>Bioassay</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>Ileum</subject><subject>Leukemia</subject><subject>Leukemia - analysis</subject><subject>Leukotrienes</subject><subject>Lipoxygenase - metabolism</subject><subject>Rats</subject><subject>Sequence analysis</subject><subject>Soybeans</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>SRS-A</subject><subject>Sulfur</subject><subject>Ultraviolet spectrum</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><recordid>eNptkMFv0zAUhy3EVErhisQBySduyZ4TJ06QOIwC20QlpBbOluM-bx5J3NkOsP-eRO2qIu1kyb_f9579EfKGQcpA5Oe7XoVUiJSnGTD-jMwZ1CwpeQ3PyRwgE0nFM_6CvAzhDgDqooIZmZUM8oJnc_JthcMvF73FHunnD_SCblr3h65R6Wj7G7oZmhBVr5Ea7zq6VpF-UsHtbm1rNZ1g7KyiS2zb8IqcGdUGfH04F-Tn1y8_llfJ6vvl9fJilWheFDERhWp4xfOCCc1rk2W1EsZUGc8Fq8tSK5HrEhHAVHy8bppyi0KXyoDeQl7V-YJ83M_dDU2HW4199KqVO2875R-kU1b-n_T2Vt643zLnVcnZyL8_8N7dDxii7GzQ4w9Uj24IUhSjw6yGsZjui9q7EDya4w4GcrIvJ_tSCMnlZH8E3p2-7Fg_6D7ZPHGP6ZGXZmjbiH_jyaAni2P-dp_fhej8sVAVo55_9FChGg</recordid><startdate>19800401</startdate><enddate>19800401</enddate><creator>Orning, Lars</creator><creator>Hammarstrom, Sven</creator><creator>Samuelsson, Bengt</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19800401</creationdate><title>Leukotriene D: A Slow Reacting Substance from Rat Basophilic Leukemia Cells</title><author>Orning, Lars ; Hammarstrom, Sven ; Samuelsson, Bengt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-75ab4843517c49f229a7ff824371966ca73c6ee00f84f82bb6de7c6af0cd03893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Amino acids</topic><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Arachidonic Acids - analysis</topic><topic>Autacoids - analysis</topic><topic>Basophils - analysis</topic><topic>Bioassay</topic><topic>Enzymes</topic><topic>Ethanol</topic><topic>Ileum</topic><topic>Leukemia</topic><topic>Leukemia - analysis</topic><topic>Leukotrienes</topic><topic>Lipoxygenase - metabolism</topic><topic>Rats</topic><topic>Sequence analysis</topic><topic>Soybeans</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>SRS-A</topic><topic>Sulfur</topic><topic>Ultraviolet spectrum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Orning, Lars</creatorcontrib><creatorcontrib>Hammarstrom, Sven</creatorcontrib><creatorcontrib>Samuelsson, Bengt</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orning, Lars</au><au>Hammarstrom, Sven</au><au>Samuelsson, Bengt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leukotriene D: A Slow Reacting Substance from Rat Basophilic Leukemia Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1980-04-01</date><risdate>1980</risdate><volume>77</volume><issue>4</issue><spage>2014</spage><epage>2017</epage><pages>2014-2017</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>A slow reacting substance produced by rat basophilic leukemia cells, treated with ionophore A23187, was characterized by spectroscopic methods, enzymatic conversions, and chemical degradations as 5-hydroxy-6-S-cysteinylglycyl-7,9,11,14-eicosatetraenoic acid (leukotriene D). γ -Glutamyltranspeptidase [γ -glutamyltransferase; (5-glutamyl)-peptide: amino-acid 5-glutamyltransferase, EC 2.3.2.2] converted leukotriene C to a product identical to leukotriene D. This suggests that the stereochemistry of the arachidonyl moiety of leukotrienes C and D is the same [5(S)-6(R)-7,9-trans-11,14-cis]. Leukotriene D induces a faster contraction and, on a molar basis, is more potent than leukotriene C in the isolated guinea pig ileum bioassay.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6103542</pmid><doi>10.1073/pnas.77.4.2014</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
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source PubMed Central; JSTOR
subjects Amino acids
Amino Acids - analysis
Animals
Arachidonic Acids - analysis
Autacoids - analysis
Basophils - analysis
Bioassay
Enzymes
Ethanol
Ileum
Leukemia
Leukemia - analysis
Leukotrienes
Lipoxygenase - metabolism
Rats
Sequence analysis
Soybeans
Spectrophotometry, Ultraviolet
SRS-A
Sulfur
Ultraviolet spectrum
title Leukotriene D: A Slow Reacting Substance from Rat Basophilic Leukemia Cells
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