Genetic Analysis of Epidermal Growth Factor Action: Assignment of Human Epidermal Growth Factor Receptor Gene to Chromosome 7

Purified murine epidermal growth factor (EGF) binds to mouse and human cells. Two mouse transformed cell lines of different origins, PG19 and B82, were found to lack EGF receptors (EGFR). The defect in each of these two cell lines seems to be identical because they fail to complement each other. Som...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1980-07, Vol.77 (7), p.4188-4192
Main Authors: Davies, Robin L., Grosse, Virginia A., Kucherlapati, Raju, Bothwell, Mark
Format: Article
Language:English
Subjects:
Animals
Cell lines
Chromosomes
Chromosomes, Human, 6-12 and X
Diploidy
Epidermal Growth Factor - metabolism
ErbB Receptors
Genes
Genes, Regulator
Genetic Linkage
Human chromosomes
Humans
Hybrid Cells
Hybridity
L cells
Membrane Proteins - genetics
Mice
Peptides - metabolism
Receptors
Receptors, Cell Surface - genetics
Somatic cells
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Description
Summary:Purified murine epidermal growth factor (EGF) binds to mouse and human cells. Two mouse transformed cell lines of different origins, PG19 and B82, were found to lack EGF receptors (EGFR). The defect in each of these two cell lines seems to be identical because they fail to complement each other. Somatic cell hybrids between these EGFR-deficient mouse cells and human cells expressing EGFR were produced. Several of these hybrids bound labeled EGF. Detailed cytogenetic analysis of these cell hybrids followed by correlation of EGFR expression with human chromosomes revealed that EGFR presence correlated with human chromosome 7. The results suggest that the structural gene or a gene necessary for expression of the human EGF receptor is located on human chromosome 7.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.7.4188