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Fluorescence Immunoassay Based on Long Time Correlations of Number Fluctuations
We report the development of a fluorescence-based immunoassay technique relying on the physical phenomena of random number fluctuations and diffusion, which we review. By determining the autocorrelation of the fluctuations in the fluorescent intensity, this method is able to measure the amount of la...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1980-08, Vol.77 (8), p.4904-4908 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c455t-dca4dc6b36ea54dfb1f8da2215f05e0531973411a63e2cf86d380a20b7fad64e3 |
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| container_end_page | 4908 |
| container_issue | 8 |
| container_start_page | 4904 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 77 |
| creator | Nicoli, D. F. Briggs, J. Elings, V. B. |
| description | We report the development of a fluorescence-based immunoassay technique relying on the physical phenomena of random number fluctuations and diffusion, which we review. By determining the autocorrelation of the fluctuations in the fluorescent intensity, this method is able to measure the amount of labeled antigen or antibody that is bound to micrometer-sized carrier particles in solution. The principal advantage of this technique is its insensitivity to small, fast-diffusing sources. It also discriminates against weakly fluorescent contaminants of size comparable to the carrier particles. We demonstrate these attributes by using two model systems: a human IgG assay and an idealized system consisting of polystyrene fluorescent spheres and rhodamine dye. |
| doi_str_mv | 10.1073/pnas.77.8.4904 |
| format | article |
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F. ; Briggs, J. ; Elings, V. B.</creator><creatorcontrib>Nicoli, D. F. ; Briggs, J. ; Elings, V. B.</creatorcontrib><description>We report the development of a fluorescence-based immunoassay technique relying on the physical phenomena of random number fluctuations and diffusion, which we review. By determining the autocorrelation of the fluctuations in the fluorescent intensity, this method is able to measure the amount of labeled antigen or antibody that is bound to micrometer-sized carrier particles in solution. The principal advantage of this technique is its insensitivity to small, fast-diffusing sources. It also discriminates against weakly fluorescent contaminants of size comparable to the carrier particles. We demonstrate these attributes by using two model systems: a human IgG assay and an idealized system consisting of polystyrene fluorescent spheres and rhodamine dye.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.77.8.4904</identifier><identifier>PMID: 7001468</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Antibodies ; Antigen-Antibody Complex ; Autocorrelation ; Charge carriers ; Fluorescence ; Fluorescent Antibody Technique ; Immunoassay ; Immunoassay - methods ; Immunoglobulin G - analysis ; Immunology ; Molecules ; Particle diffusion ; Particle intensity ; Particle motion ; Spectrometry, Fluorescence</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1980-08, Vol.77 (8), p.4904-4908</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-dca4dc6b36ea54dfb1f8da2215f05e0531973411a63e2cf86d380a20b7fad64e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/77/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/9217$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/9217$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,53789,53791,58236,58469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7001468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nicoli, D. F.</creatorcontrib><creatorcontrib>Briggs, J.</creatorcontrib><creatorcontrib>Elings, V. B.</creatorcontrib><title>Fluorescence Immunoassay Based on Long Time Correlations of Number Fluctuations</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We report the development of a fluorescence-based immunoassay technique relying on the physical phenomena of random number fluctuations and diffusion, which we review. By determining the autocorrelation of the fluctuations in the fluorescent intensity, this method is able to measure the amount of labeled antigen or antibody that is bound to micrometer-sized carrier particles in solution. The principal advantage of this technique is its insensitivity to small, fast-diffusing sources. It also discriminates against weakly fluorescent contaminants of size comparable to the carrier particles. We demonstrate these attributes by using two model systems: a human IgG assay and an idealized system consisting of polystyrene fluorescent spheres and rhodamine dye.</description><subject>Antibodies</subject><subject>Antigen-Antibody Complex</subject><subject>Autocorrelation</subject><subject>Charge carriers</subject><subject>Fluorescence</subject><subject>Fluorescent Antibody Technique</subject><subject>Immunoassay</subject><subject>Immunoassay - methods</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunology</subject><subject>Molecules</subject><subject>Particle diffusion</subject><subject>Particle intensity</subject><subject>Particle motion</subject><subject>Spectrometry, Fluorescence</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><recordid>eNp9kc1P3DAQxS1ERRfaKxKHSj5xSzqO7dg59EBXfEmrcqFny0nGEJTEWzup4L_Hq12tlgsna_x-b2Y0j5BzBjkDxX-uRxtzpXKdiwrEEVkwqFhWpuKYLAAKlWlRiK_kNMYXAKikhhNyogCYKPWCPNz0sw8YGxwbpPfDMI_exmjf6G8bsaV-pCs_PtHHbkC69CFgb6fOj5F6R__MQ42BphbNNG-_v5EvzvYRv-_eM_L35vpxeZetHm7vl1errBFSTlnbWNE2Zc1LtFK0rmZOt7YomHQgESRnleKCMVtyLBqny5ZrsAXUytm2FMjPyK9t3_VcD9im9adge7MO3WDDm_G2Mx-VsXs2T_6_4aKqpEr-y50_-H8zxskMXTpC39sR_RyNklyk-TqB-RZsgo8xoNvPYGA2CZhNAkYpo80mgWT4cbjZHt-d_EDf-Pbqgf_yM924ue8nfJ0SeLEFX-Lkw56sCqb4O9dapJo</recordid><startdate>19800801</startdate><enddate>19800801</enddate><creator>Nicoli, D. 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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1980-08, Vol.77 (8), p.4904-4908 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_77_8_4904_fulltext |
| source | JSTOR Archival Journals and Primary Sources Collection【Remote access available】; NCBI_PubMed Central（免费） |
| subjects | Antibodies Antigen-Antibody Complex Autocorrelation Charge carriers Fluorescence Fluorescent Antibody Technique Immunoassay Immunoassay - methods Immunoglobulin G - analysis Immunology Molecules Particle diffusion Particle intensity Particle motion Spectrometry, Fluorescence |
| title | Fluorescence Immunoassay Based on Long Time Correlations of Number Fluctuations |
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