Immunoreactive Dynorphin in Mammalian Spinal Cord and Dorsal Root Ganglia

The distribution of immunoreactive dynorphin (ir-dynorphin) has been determined in dorsal and ventral aspects of spinal cord and in dorsal root ganglia of rabbit and rat. Concentrations are highest in dorsal cord, with intermediate levels in ventral cord and low levels in dorsal root ganglia of both...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-12, Vol.78 (12), p.7783-7786
Main Authors: Botticelli, Lawrence J., Cox, Brian M., Goldstein, Avram
Format: Article
Language:English
Subjects:
Animals
Anterior hypothalamus
Dynorphins
Endorphins - immunology
Endorphins - metabolism
Ganglia, Spinal - metabolism
Gel chromatography
Neurons
Neurotransmitter Agents - metabolism
Pain - physiopathology
Rabbits
Radioimmunoassay
Rats
Rhizotomy
Spinal cord
Spinal Cord - metabolism
Spinal ganglia
Spinal Nerve roots
Vertebrae
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Summary:The distribution of immunoreactive dynorphin (ir-dynorphin) has been determined in dorsal and ventral aspects of spinal cord and in dorsal root ganglia of rabbit and rat. Concentrations are highest in dorsal cord, with intermediate levels in ventral cord and low levels in dorsal root ganglia of both species. Levels of ir-dynorphin are relatively uniform over examined segments (vertebrae C2-S3) of rabbit spinal cord and dorsal root ganglia. Gel permeation chromatography of extracts from rabbit dorsal and ventral spinal cord and dorsal root ganglia revealed at least three immunoreactive components of differing molecular size in all three structures. Multiple unilateral or bilateral dorsal rhizotomy (vertebrae C5-T1) in rat did not affect levels of ir-dynorphin in spinal cord. As reported [Goldstein, A. & Ghazarossian, V. E. (1980) Proc. Natl. Acad. Sci. USA 77, 6207-6210], midthoracic spinal transection was without effect. Within the spinal cord, the neuropeptide appears, most probably, to be contained in shortaxoned neurons. We surmise that this potent opioid peptide may participate in the processing of sensory information in spinal cord.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.12.7783