Multiple Biologic Activities of a Cloned Inducer T-Cell Population

• Published in: Proceedings of the National Academy of Sciences - PNAS 1981-02, Vol.78 (2), p.1157-1161
• Main Authors: Nabel, Gary, Greenberger, Joel S., Sakakeeny, Mary Ann, Cantor, Harvey
• Format: Article
• Language: English
• Subjects: Animals; B lymphocytes; B-Lymphocytes - immunology; Bone marrow cells; Cell cloning; Cell culture techniques; Cells, Cultured; Clone Cells; Cultured cells; Granulocytes - immunology; Immunoglobulins - biosynthesis; Immunology; Interleukin-1; Macrophages - immunology; Mast cells; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Proteins - immunology; Secretion; Spleen cells; T lymphocytes; T-Lymphocytes - immunology
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.78.2.1157

The mouse T-cell clone Lyl+2-/9, belonging to the Ly1 set, displays the following functions in vitro: (i) augmentation of immunoglobulin output by B cells; (ii) stimulation of bone marrow cells to produce colonies composed of granulocytes, macrophages, or both; and (iii) proliferative stimulation of T-cell clones belonging to other Ly sets. These functions are induced by Lyl+2-/9 cells themselves and by supernatants of Lyl+2-/9 cultures and are not evinced by tested clones belonging to other Ly sets. The agent or agents responsible for colony formation and for B-cell stimulation had an apparent molecular weight of 45,000-50,000 and could not be physically separated. The T-cell stimulating agents(s) had an apparent molecular weight of 30,000 and could be separated from the agent(s) that acts upon colony formation and B cells. Thus, clone Lyl+2-/9 produces at least two soluble products that induce or augment activities of at least three other differently programmed cell sets.