Differential effects of transforming avian RNA tumor viruses on avian macrophages [Rous sarcoma virus]

Functionally differentiated chicken macrophages were derived by in vitro differentiation of embryonic yolk sac cells and were characterized by several macrophage-specific cell markers. Uniform, infected, virus-producing cultures were obtained after exposure of these macrophages to avian myoblastosis...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-06, Vol.78 (6), p.3600-3604
Main Authors: Durbn, E.M, Boettiger, D
Format: Article
Language:English
Subjects:
Acid Phosphatase - metabolism
Alpharetrovirus - physiology
Animals
Cell Adhesion
Cell Transformation, Viral
Cells
Cellular differentiation
Chick Embryo
Chickens
Fibroblasts
Giant cells
Infections
Macrophages
Macrophages - cytology
Macrophages - microbiology
Phagocytes
Phagocytosis
Stem cells
T lymphocytes
Viruses
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Summary:Functionally differentiated chicken macrophages were derived by in vitro differentiation of embryonic yolk sac cells and were characterized by several macrophage-specific cell markers. Uniform, infected, virus-producing cultures were obtained after exposure of these macrophages to avian myoblastosis virus (AMV), avian myelocytomatosis virus (MC29), myeloblastosis-associated virus (MAV-2), and Prague strain of Rous sarcoma virus (PR-B RSV). Both AMV and MC29 induced morphological transformation typical of the in vivo leukemias induced by these virus strains. Analysis of the expression of macrophage-specific markers in these two transformed cell types demonstrated that different markers of the mature macrophage were suppressed by each virus, even though the parental cell immediately preceding the transformation event was a mature macrophage in both cases. Cells infected with PR-B RSV and MAV-2 showed no observable difference from uninfected macrophages in terms of morphological characteristics, growth rate, or expression of the differentiated functions of macrophages. This system provides demonstrations of a cell type that produces infectious, transforming RSV but fails to respond by functional alterations induced by the transforming gene, src.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.6.3600