Cloning and Analysis of Strong Promoters is Made Possible by the Downstream Placement of a RNA Termination Signal

Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-08, Vol.78 (8), p.4936-4940
Main Authors: Gentz, Reiner, Langner, Annette, Annie C. Y. Chang, Cohen, Stanley N., Bujard, Hermann
Format: Article
Language:English
Subjects:
Bacteriophages
Base Sequence
Cloning, Molecular - methods
DNA
Gene Expression Regulation
Genes
Genes, Bacterial
Genes, Viral
Lac operon
Operator regions
Operon
Phage T5
Plasmids
Promoter regions
RNA
T-Phages - genetics
Terminator codon
Terminator regions
Transcription, Genetic
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Description
Summary:Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of promoters and terminators, respectively. By assessing transcription within the indicator regions, the efficiency of promoters as well as termination signals can be determined in vitro and in vivo.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.8.4936