Immunocytochemical localization of tubulin, actin, and myosin in axonemes of ciliated cells from quail oviduct

Tubulin, actin, and myosin have been localized in isolated demembranated ciliated cells from quail oviduct by immunocytochemistry in both light and electron microscopy by using purified antibodies. The peripheral doublets and the central tubules are stained by the antitubulin whereas the kinetosomes...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982-05, Vol.79 (10), p.3198-3202
Main Authors: Sandoz, Daniel, Gounon, Pierre, Karsenti, Eric, Sauron, Marie-Elizabeth
Format: Article
Language:English
Subjects:
Actins
Actins - metabolism
Aldehydes
Animals
Antibodies
Cells
Cilia
Cilia - metabolism
Cilia - ultrastructure
Coturnix
Epithelial cells
Female
Frozen sections
Immunocytochemistry
Immunoenzyme Techniques
Microvilli
Myosins - metabolism
Oviducts
Oviducts - metabolism
Oviducts - ultrastructure
Tubulin - metabolism
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Summary:Tubulin, actin, and myosin have been localized in isolated demembranated ciliated cells from quail oviduct by immunocytochemistry in both light and electron microscopy by using purified antibodies. The peripheral doublets and the central tubules are stained by the antitubulin whereas the kinetosomes are poorly stained. Actin antibodies clearly stain the axonemes, but only on the proximal-half portion, whereas myosin antibodies stain a small area of the axonemes just above the ciliary neck region.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.10.3198