Preparation of a Monoclonal Antibody to Rat Liver Glucose-6-phosphate Dehydrogenase and the Study of Its Immunoreactivity with Native and Inactivated Enzyme

A monoclonal antibody of the IgG class was prepared against rat liver glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate:NADP+1-oxidoreductase, EC 1.1.1.49) by the hybridoma technique. This antibody does not affect the catalytic activity of the enzyme and shows crossreactivity with the p...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982-05, Vol.79 (9), p.2860-2864
Main Authors: Dao, My Lien, Johnson, B. Connor, Hartman, Philip E.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal
Antigen-Antibody Reactions
Antiserum
Binding, Competitive
Cross Reactions
Dehydrogenases
Diet
Enzymes
Glucosephosphate Dehydrogenase - antagonists & inhibitors
Glucosephosphate Dehydrogenase - immunology
Hybridomas
Liver
Liver - enzymology
Monoclonal antibodies
Precipitins
Protein Conformation
Rats
Structure-Activity Relationship
Ungulates
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Description
Summary:A monoclonal antibody of the IgG class was prepared against rat liver glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate:NADP+1-oxidoreductase, EC 1.1.1.49) by the hybridoma technique. This antibody does not affect the catalytic activity of the enzyme and shows crossreactivity with the palmitoyl CoA-inactivated G6PD. By solid phase radioimmunoassay, the presence of crossreacting materials in comparable amounts was determined in liver homogenate supernatants from rats that had been starved and refed a high-sucrose diet (containing a high level of G6PD activity) and from rats that had been starved and refed a high-fat diet (containing a low level of G6PD activity). These findings indicate that G6PD is present in an inactive form in rats fed a high-fat diet. The monoclonal antibody will facilitate isolation and characterization of the inactive variant G6PD.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.9.2860