Cloning and Sequence Analysis of cDNA for the Precursor of Human Growth Hormone-Releasing Factor, Somatocrinin

Molecular cloning has established the primary structures of two precursors of the human pancreas growth hormone-releasing factor (hpGRF-44), somatocrinin. Both polypeptides contain the sequence of hpGRF-44 flanked by basic processing sites. Furthermore, the precursors include a putative signal seque...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-07, Vol.80 (14), p.4311-4314
Main Authors: Gubler, Ueli, Monahan, John J., Lomedico, Peter T., Bhatt, Ram S., Collier, Kenneth J., Hoffman, Beth J., Böhlen, Peter, Esch, Frederick, Ling, Nicholas, Zeytin, Füsun, Brazeau, Paul, Poonian, Mohindar S., Gage, L. Patrick
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Base Sequence
Biochemistry
Cloning, Molecular
Complementary DNA
DNA - analysis
DNA, Neoplasm - genetics
Gels
Growth Hormone-Releasing Hormone - genetics
Humans
Messenger RNA
Nucleic Acid Hybridization
Nucleotides
Pancreatic Neoplasms - genetics
Peptide Fragments - genetics
Poly A - genetics
Protein Biosynthesis
RNA
RNA - genetics
RNA, Messenger - genetics
Sequence analysis
Three prime untranslated regions
Tumors
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Summary:Molecular cloning has established the primary structures of two precursors of the human pancreas growth hormone-releasing factor (hpGRF-44), somatocrinin. Both polypeptides contain the sequence of hpGRF-44 flanked by basic processing sites. Furthermore, the precursors include a putative signal sequence and a carboxyl-terminal amidation signal for hpGRF-44. The two forms of mRNA code for pre-pro-GRF-107 and pre-pro-GRF-108. Pre-pro-GRF-108 differs from pre-pro-GRF-107 by the insertion of a serine in the carboxyl-terminal portion of the precursor. In vitro translation of tumor poly(A)+ RNA followed by immunoprecipitation with GRF-specific antiserum and gel electrophoresis showed the molecular weight of preprosomatocrinin to be approximately 13,000, which is in good agreement with the molecular weight deduced from the sequences of the cDNA clones.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.14.4311