In vitro transcription of a silkworm 5S RNA gene requires an upstream signal

The RNA polymerase III transcription complex from Bombyx mori silkworms, in contrast to that from Xenopus frogs, requires a signal that is upstream from the transcription initiation site. We have found that part of the normal 5′flanking sequence is essential for transcription of Bombyx 5S RNA genes...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1984-09, Vol.81 (17), p.5519-5522
Main Authors: Morton, Diane G., Sprague, Karen U.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Bombyx - enzymology
Bombyx - genetics
Chromosome Deletion
Conserved sequences
DNA
DNA Restriction Enzymes
DNA-Directed RNA Polymerases - metabolism
Gels
Genes
Mutation
Nucleotide sequences
Nucleotides
Ovaries
RNA
RNA Polymerase III - metabolism
RNA, Ribosomal - genetics
Silkworms
Transcription, Genetic
Transfer RNA
Xenopus
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Summary:The RNA polymerase III transcription complex from Bombyx mori silkworms, in contrast to that from Xenopus frogs, requires a signal that is upstream from the transcription initiation site. We have found that part of the normal 5′flanking sequence is essential for transcription of Bombyx 5S RNA genes by homologous cell-free extracts. The critical region is structurally similar to the upstream signal that directs Bombyx tRNA gene transcription. The association of such a control element with two classes of polymerase III templates argues that it acts through a general component of the Bombyx transcription apparatus.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.81.17.5519