Regulated Expression of a Human Interferon Gene in Yeast: Control by Phosphate Concentration or Temperature

The promoter/regulator region from the yeast repressible acid phosphatase gene was used to construct a vector for the regulated expression of cloned genes in yeast. The gene for human leukocyte interferon was inserted into this vector. Yeast cells transformed with the resulting plasmid produced sign...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1984-01, Vol.81 (2), p.367-370
Main Authors: Kramer, Richard A., DeChiara, Thomas M., Schaber, Michael D., Hilliker, Sandra
Format: Article
Language:English
Subjects:
Acid Phosphatase - genetics
DNA
DNA, Fungal - genetics
DNA, Recombinant
gene expression
Gene Expression Regulation
gene regulation
Genes
Genetic Vectors
Humans
interferon
Interferon Type I - genetics
Interferons
man
Operon
phosphate
Phosphates
Plasmids
Promoter regions
RNA
Saccharomyces cerevisiae - genetics
Start codon
Temperature
Yeasts
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Description
Summary:The promoter/regulator region from the yeast repressible acid phosphatase gene was used to construct a vector for the regulated expression of cloned genes in yeast. The gene for human leukocyte interferon was inserted into this vector. Yeast cells transformed with the resulting plasmid produced significant amounts of interferon only when grown in medium lacking inorganic phosphate. Mutants in two acid phosphatase regulatory genes (coding for a defective repressor and a temperature-sensitive positive regulator) were used to develop a yeast strain that grew well at a high temperature (35 degrees C) but produced interferon only at a low temperature (23 degrees C), independent of phosphate concentration.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.81.2.367