Interleukin 2 (IL-2) Augments Transcription of the IL-2 Receptor Gene

We demonstrate that purified interleukin 2 (IL-2) can directly upregulate IL-2 receptor expression on phytohemagglutinin-activated T lymphocytes maintained in culture until IL-2 receptor expression had markedly declined. The IL-2-induced increase in IL-2 receptor number is maximal within 12 hr, requ...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-06, Vol.82 (12), p.4230-4234
Main Authors: Depper, Joel M., Leonard, Warren J., Drogula, Cynthia, Kronke, Martin, Waldmann, Thomas A., Greene, Warner C.
Format: Article
Language:English
Subjects:
Antigens
Biological and medical sciences
Cells, Cultured
Cultured cells
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation - drug effects
Genes
Humans
Interleukin-2 - pharmacology
Lymphocytes
Lymphocytes - physiology
Messenger RNA
Molecular and cellular biology
Molecular genetics
Oncogenes
Phytohemagglutinins - pharmacology
Protein Biosynthesis
Receptors
Receptors, Cell Surface - genetics
Receptors, Immunologic - genetics
Receptors, Immunologic - metabolism
Receptors, Interleukin-2
Receptors, Transferrin
RNA
RNA, Messenger - genetics
T lymphocytes
Tetradecanoylphorbol Acetate - pharmacology
Transcription, Genetic - drug effects
Transferrin receptors
Up regulation
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Summary:We demonstrate that purified interleukin 2 (IL-2) can directly upregulate IL-2 receptor expression on phytohemagglutinin-activated T lymphocytes maintained in culture until IL-2 receptor expression had markedly declined. The IL-2-induced increase in IL-2 receptor number is maximal within 12 hr, requires new RNA and protein synthesis, and is mediated by an interaction of ligand with the high-affinity receptors for IL-2. IL-2 stimulation results in increased accumulation of IL-2 receptor mRNA within 4 hr, while an increase in IL-2 receptor gene transcription is detected within 30 min in isolated nuclei. In addition, IL-2 incubation results in increased amounts of c-myc and transferrin receptor mRNA, but it does not augment levels of mRNA encoding the β chain of the T-cell receptor for antigen. These results demonstrate that IL-2 can directly upregulate transcription and expression of its own receptor and, therefore, indicate that IL-2 may regulate IL-2-dependent immune responses, in part, by influencing the expression of IL-2 receptors.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.12.4230