Human Seminal α -Inhibins: Detection in Human Pituitary, Hypothalamus, and Serum by Immunoreactivity

An antiserum generated in rabbits against human seminal α -inhibin-52 has been used to develop a sensitive radioimmunoassay for the detection of α -inhibins. The α -inhibin-52 antiserum reacts with α -inhibin-92 and α -inhibin-31 with equal avidity. These peptides were found to be present in human p...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-05, Vol.83 (10), p.3484-3486
Main Authors: Ramasharma, K., Li, Choh Hao
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Antibodies
Antiserum
Biological and medical sciences
Cellulose nitrate
Chromatography
Endocrinology
Fundamental and applied biological sciences. Psychology
Gels
Hormones
Humans
Hypothalamus
Hypothalamus - metabolism
Immunosorbent Techniques
Inhibins - blood
Inhibins - metabolism
Molecules
Pituitary Gland - metabolism
Proteins
Radioimmunoassay
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Summary:An antiserum generated in rabbits against human seminal α -inhibin-52 has been used to develop a sensitive radioimmunoassay for the detection of α -inhibins. The α -inhibin-52 antiserum reacts with α -inhibin-92 and α -inhibin-31 with equal avidity. These peptides were found to be present in human pituitary, hypothalamus, and serum. In exclusion chromatography on Sephadex G-100, the immunoreactive material eluted in a large molecular size region. Immunoblot analysis of column-derived fractions of these extracts revealed the presence of α -inhibin-92. The mean concentrations of immunoreactive α -inhibin were found to be 7.2 ng/ml in normal adult male serum, 70.3 ng/g (wet weight) of pituitary, and 12.9 ng/g (wet weight) of hypothalamus. This communication reports on the evidence for the existence of gonadal peptides in the brain.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.10.3484