Characterization of a Female-Specific cDNA Derived from a Developmentally Regulated mRNA in the Human Blood Fluke Schistosoma mansoni

We have isolated and characterized a cDNA clone that is derived from a developmentally regulated mRNA found only in mature female schistosomes. The mRNA is approximately 950 nucleotides in length and is not detectable in immature female schistosomes isolated from single-sex infections, in male worms...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-08, Vol.83 (15), p.5544-5548
Main Authors: Bobek, Libuse, Rekosh, David M., Van Keulen, Harry, LoVerde, Philip T.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Bisexuality
Cloning, Molecular
Complementary DNA
DNA - genetics
Female
Female animals
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genetic engineering
Genetic technics
Immatures
Infections
Male animals
Messenger RNA
Methods. Procedures. Technologies
Molecular cloning
Nucleic Acid Hybridization
Parasitism
RNA
RNA, Messenger - genetics
Schistosoma mansoni
Schistosoma mansoni - genetics
Schistosoma mansoni - growth & development
Worms
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Summary:We have isolated and characterized a cDNA clone that is derived from a developmentally regulated mRNA found only in mature female schistosomes. The mRNA is approximately 950 nucleotides in length and is not detectable in immature female schistosomes isolated from single-sex infections, in male worms, or in eggs. During normal bisexual infections, the mRNA species is first detected 28 days after infection (the time of worm pairing) and increases to a high level 35 days after infection, coinciding with the start of egg production. The nucleotide sequence of the cDNA shows two large open reading frames in the coding strand. Several features of the clone, including the deduced sequence of the polypeptide encoded by one of the reading frames, suggest a relationship to the silk moth chorion (egg shell) gene family. The isolation of this clone provides us with a probe for further studies of female schistosome development and is a first step toward a detailed understanding of this process at the molecular level.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.15.5544