Use of Monosomics to Map Cloned DNA Fragments in Maize

Monosomic maize (Zea mays L.) plants were generated using the r-X1 deficiency system, and the monosomy was confirmed both genetically and cytologically. Genomic DNAs prepared from a group of plants, each monosomic for one chromosome, were digested with restriction enzymes, electrophoresed in agarose...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-08, Vol.83 (16), p.6035-6039
Main Authors: Helentjaris, Tim, Weber, David F., Wright, Scott
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Sciences: Genetics
Chromosomes
Corn
Diploidy
DNA
Fundamental and applied biological sciences. Psychology
Genes. Genome
Genetic loci
Genomics
Linkage groups
Mendelian inheritance
Molecular and cellular biology
Molecular genetics
Monosomics
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Summary:Monosomic maize (Zea mays L.) plants were generated using the r-X1 deficiency system, and the monosomy was confirmed both genetically and cytologically. Genomic DNAs prepared from a group of plants, each monosomic for one chromosome, were digested with restriction enzymes, electrophoresed in agarose gels, and blotted onto nylon membranes. Hybridization of labeled cloned DNA fragments to these blots proved efficient in assigning each fragment to the chromosome from which it originated. Cloned DNA has previously contributed to loci detection through the use of the restriction fragment length polymorphisms (RFLPs), these loci subsequently being arranged into linkage groups by segregation analysis. In this study, these linkage groups were assigned to specific chromosomes, facilitating the construction of a linkage map for maize containing 112 RFLP loci. An additional 35 loci were also assigned to chromosomes by this method; however, the linkage relationships of these loci to other RFLP loci on each chromosome remains undetermined.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.16.6035