An Epstein-Barr Virus (EBV)-Determined Nuclear Antigen (EBNA5) Partly Encoded by the Transformation-Associated Bam WYH Region of EBV DNA: Preferential Expression in Lymphoblastoid Cell Lines

• Published in: Proceedings of the National Academy of Sciences - PNAS 1986-09, Vol.83 (17), p.6641-6645
• Main Authors: Dillner, Joakim, Kallin, Bengt, Alexander, Hannah, Ernberg, Ingemar, Uno, Masatsune, Ono, Yasushi, Klein, George, Lerner, Richard A.
• Format: Article
• Language: English
• Subjects: Amino acids; Antibodies; Antibodies, Viral - immunology; Antigens, Viral - genetics; B cell lymphoma; B lymphocytes; Biological and medical sciences; Burkitt lymphoma; Burkitt Lymphoma - microbiology; Cell Line; Cell lines; Cell Nucleus - physiology; Cell Transformation, Viral; Complementary DNA; DNA, Viral - genetics; Epstein Barr virus infections; Epstein-Barr Virus Nuclear Antigens; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation; Genes, Viral; Herpesvirus 4, Human - genetics; Humans; Infections; Lymphocytes - physiology; Molecular and cellular biology; Molecular genetics; Palatine Tonsil - microbiology; Peptide Fragments - chemical synthesis; Peptide Fragments - immunology; Viral Proteins - genetics; Viruses
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.83.17.6641

Four peptides were synthesized on the basis of amino acid sequences deduced from a highly spliced transcript encoded by the Bam W, Y, and H fragments of the Epstein-Barr virus (EBV) genome [Bodescot, M., Chambraud, J. B., Farrell, P. J. & Perricaudet, M. (1984) EMBO J. 3, 1913-1917]. Rabbit antisera against three of the four peptides identified a nuclear polypeptide that varied between 22 and 70 kDa in molecular size. Four of 20 EBV-positive human sera contained antibodies against this polypeptide. Since this is the fifth EBV-determined nuclear antigen (EBNA) discovered in growth-transformed cells, it is designated EBNA5. The antigen was detected in virus nonproducer lines (