Effects of Glucagon and Vasopressin on Hepatic Ca2+ Release

The effects of physiological levels of glucagon on Ca2+ efflux were examined in the perfused rat liver. Two methods were used to estimate Ca2+ efflux: (i) prior labeling of the calcium pools with 45Ca2+ and (ii) measurement of perfusate Ca2+ with atomic absorption. According to both methods, glucago...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-12, Vol.83 (23), p.8943-8946
Main Author: Kraus-Friedmann, Naomi
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biochemistry
Biological and medical sciences
Calcium - metabolism
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology
Drug Interactions
Endoplasmic reticulum
Fundamental and applied biological sciences. Psychology
Glucagon - pharmacology
Hepatocytes
Hormones
Inorganic compounds
Liver
Liver - metabolism
Male
Microsomes, Liver - metabolism
Mitochondria
Mitochondria, Liver - metabolism
Other biological molecules
Rats
Vasopressins - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effects of physiological levels of glucagon on Ca2+ efflux were examined in the perfused rat liver. Two methods were used to estimate Ca2+ efflux: (i) prior labeling of the calcium pools with 45Ca2+ and (ii) measurement of perfusate Ca2+ with atomic absorption. According to both methods, glucagon administration at the physiological level evoked Ca2+ release. The released Ca2+ originated mostly from a carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP)-depletable pool and also from an FCCP-insensitive pool from which Ca2+ could be released with A23187. Maximally effective doses of glucagon and vasopressin had no additive effect on Ca2+ release. Prior administration of vasopressin resulted in markedly reduced Ca2+ release by glucagon. These results indicate that glucagon releases Ca2+ from the same pool that vasopressin does.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.23.8943