Temporal and Tissue-Specific Expression of Mouse ets Genes

• Published in: Proceedings of the National Academy of Sciences - PNAS 1987-05, Vol.84 (10), p.3161-3165
• Main Authors: Bhat, Narayan K., Fisher, Robert J., Fujiwara, Shigeyoshi, Ascione, Richard, Papas, Takis S.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cell cycle; Cell growth; Chromosomes; DNA; DNA Replication; Fundamental and applied biological sciences. Psychology; Gene expression; Genes; Genetic loci; Hepatectomy; Liver; Liver - analysis; Liver Regeneration; Male; Messenger RNA; Mice; Mice, Inbred BALB C; Molecular and cellular biology; Molecular genetics; Molecular Weight; Oncogenes; Plasmids; RNA; RNA, Messenger - genetics; RNA, Messenger - isolation & purification; Tissue Distribution; Transcription, Genetic
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.84.10.3161

The expression of ets genes has been studied in mouse tissues and regenerating murine liver, an in vivo model for cell proliferation. Our results indicate that (i) the ets-1 and ets-2 loci are transcriptionally active; (ii) the ets-2 locus encodes a major mRNA (3.5 kilobases) and is expressed in most of the tissues examined, whereas the ets-1 locus encodes a major 5.3-kilobase and minor 4.0-, 2.5-, and 2.2-kilobase RNA species and is expressed at a high level in thymus; (iii) both ets-1 and ets-2 mRNA are abundant in young proliferating tissues and are greatly reduced in terminally differentiated tissues, except thymus; (iv) compensatory growth of liver induces ets-2 mRNA before DNA synthesis, but after fos and myc induction; and (v) ets-2 mRNA, but not ets-1 mRNA, is stabilized in the presence of cycloheximide during hepatic regeneration. These results suggest that ets-2 gene expression is intrinsically linked with cell proliferation. Thus, ets-2 expression follows a pattern similar to other members of the nuclear oncogene family. During hepatic regeneration, the ets-1 and ets-2 loci are subject to differential regulation.