On Extended X-Ray Absorption Fine Structure Studies of Hemoglobin

Results of extended x-ray absorption fine structure (EXAFS) studies of the iron atom in deoxygenated hemoglobin are reviewed. It is shown that the iron-porphinato nitrogen distance has been determined to be 2.06 ± 0.01 angstrom by two independent investigations [Eisenberger, P. M., Shulman, R. G., K...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-02, Vol.84 (4), p.973-974
Main Author: Shulman, R. G.
Format: Article
Language:English
Subjects:
Absorption
Biological and medical sciences
Biophysics
Chemical Phenomena
Chemistry
Fine structure
Fundamental and applied biological sciences. Psychology
Hemoglobins
Hemoglobins - metabolism
Iron
Ligands
Molecular biophysics
Nitrogen
Oxygen - metabolism
Porphyrins
Protein Conformation
Spectroscopy : techniques and spectras
Triangulation
X-Rays
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Summary:Results of extended x-ray absorption fine structure (EXAFS) studies of the iron atom in deoxygenated hemoglobin are reviewed. It is shown that the iron-porphinato nitrogen distance has been determined to be 2.06 ± 0.01 angstrom by two independent investigations [Eisenberger, P. M., Shulman, R. G., Kincaid, B. M., Brown, G. S. & Ogawa, S. (1978) Nature (London) 274, 30-34 and Perutz, M. F., Hasnain, S. S., Duke, P. J., Sessler, J. L. & Hahn, J. E. (1982) Nature (London) 295, 535-538]. Difficulties experienced by Perutz et al. in using this distance to calculate the iron's distance above the plane by triangulation are shown to be due to ignoring differences between ferrous and ferric hemes. It is concluded that the iron is 0.2 -0.2+0.1 angstrom above the plane of the nitrogens as originally shown by Eisenberger et al.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.4.973