Differential Expression of the Homeobox Gene Hox-1.3 in F9 Embryonal Carcinoma Cells

The Hox-1.3 gene is located on mouse chromosome 6 and has been previously shown to be expressed in mouse embryos and adults. In this study, we have examined the steady-state levels of the Hox-1.3 transcripts in undifferentiated and differentiated F9 embryonal carcinoma cells. We find that there is a...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-08, Vol.85 (15), p.5587-5591
Main Authors: Murphy, Shawn P., Garbern, James, Odenwald, Ward F., Lazzarini, Robert A., Linney, Elwood
Format: Article
Language:English
Subjects:
3T3 cells
Animals
Autoradiography
Biological and medical sciences
Cell Differentiation
Cell lines
Cellular differentiation
Cultured cells
Densitometry
Embryonal Carcinoma Stem Cells
Fundamental and applied biological sciences. Psychology
Gels
Gene expression
Gene Expression Regulation
Genes
Genes, Homeobox
Messenger RNA
Molecular and cellular biology
Molecular genetics
Neoplastic Stem Cells - metabolism
Neoplastic Stem Cells - pathology
NIH 3T3 cells
Nucleic Acid Hybridization
RNA
RNA - genetics
RNA probes
Teratoma
Transcription, Genetic
Tumor Cells, Cultured
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Hox-1.3 gene is located on mouse chromosome 6 and has been previously shown to be expressed in mouse embryos and adults. In this study, we have examined the steady-state levels of the Hox-1.3 transcripts in undifferentiated and differentiated F9 embryonal carcinoma cells. We find that there is a rapid increase of Hox-1.3 transcripts after differentiation induction of F9 cells. The level of the major 1.85-kilobase (kb) transcript peaks at 16-24 hr after differentiation induction of F9 cells. By using primer extension techniques the 5′ends of the major 1.85-kb transcript have been mapped to two sites in induced F9 cells. Cellular fractionation of RNA and transfer blot gel analysis has localized one minor transcript to the nucleus, whereas the major transcript and two additional minor transcripts appear in the nucleus and the cytoplasm of induced F9 cells. The results of nuclear run-off experiments with uninduced and induced F9 cell nuclei indicate that there is a substantial increase in the rate of Hox-1.3 transcription upon induction of F9 cells with retinoic acid.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.15.5587