Molecular Analysis of the 60-kDa Human Ro Ribonucleoprotein

Ro, or Sjogren syndrome type A (SS-A), antigen is the most prevalent of the human systemic autoimmune specificities and exists as an inabundant ribonucleoprotein complex (RNP) composed of a 60,649-Da protein, as defined here by cDNA cloning, and the human Y RNAs. The recombinant 60-kDa Ro protein an...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-12, Vol.85 (24), p.9479-9483
Main Authors: Deutscher, Susan L., Harley, John B., Keene, Jack D.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Analytical, structural and metabolic biochemistry
Antibodies
Antigens
Antiserum
Autoantigens - analysis
Autoantigens - genetics
Base Sequence
Biological and medical sciences
Cloning, Molecular
Complementary DNA
DNA - analysis
Fundamental and applied biological sciences. Psychology
Gels
HeLa cells
Humans
Lupus Erythematosus, Systemic - immunology
Molecular Sequence Data
Molecular Weight
Nucleic acids
Nucleotide Mapping
Peptide Mapping
Protein Biosynthesis
Ribonucleoproteins
RNA
Rna, ribonucleoproteins
RNA, Small Cytoplasmic
Sjogren's Syndrome - immunology
Ungulates
Zinc
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Summary:Ro, or Sjogren syndrome type A (SS-A), antigen is the most prevalent of the human systemic autoimmune specificities and exists as an inabundant ribonucleoprotein complex (RNP) composed of a 60,649-Da protein, as defined here by cDNA cloning, and the human Y RNAs. The recombinant 60-kDa Ro protein and human Y1 RNA were reconstituted in vitro, and the binding was enhanced by divalent cations. A region of the Ro amino acid sequence revealed a resemblance to the RNP consensus motif found in most RNA-binding proteins. In addition, Ro contained a potential ``zinc-binding finger'' motif that was distinct from the RNP consensus region and that may participate in the interaction with human Y RNAs or with other proteins. The recombinant Ro fusion protein also proved useful for the detection of autoantibodies in the sera of patients with autoimmune disorders. Possible functions of the Ro RNPs and their relationship to RNA polymerase III transcription are discussed.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.24.9479