Injection of an Antibody against a p21 c-Ha-ras Protein Inhibits Cleavage in Axolotl Eggs

The presence of a ras protein was demonstrated in cleaving axolotl eggs by selective immunoprecipitation with a polyclonal antibody against a peptide encoded by the c-Ha-ras oncogene, cellular homolog of the v-Ha-ras oncogene of Harvey rat sarcoma virus. Injection of this antibody into axolotl oocyt...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-01, Vol.85 (2), p.502-506
Main Authors: Baltus, Elyane, Hanocq-Quertier, Jacqueline, Hanocq, Françoise, Brachet, Jean
Format: Article
Language:English
Subjects:
Ambystoma
Animals
Antibodies
Biological and medical sciences
Blastocyst - cytology
Blastomeres
Cell cycle
Cell cycle, cell proliferation
Cell growth
Cell physiology
DNA replication
Eggs
Embryos
Female
Fertilization
Fundamental and applied biological sciences. Psychology
Gastrula - cytology
Meiosis
Membrane Proteins - physiology
Molecular and cellular biology
Oocytes
Oocytes - cytology
Proto-Oncogene Proteins - immunology
Proto-Oncogene Proteins - physiology
Proto-Oncogene Proteins p21(ras)
ras genes
Somatic cells
Xenopus
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Summary:The presence of a ras protein was demonstrated in cleaving axolotl eggs by selective immunoprecipitation with a polyclonal antibody against a peptide encoded by the c-Ha-ras oncogene, cellular homolog of the v-Ha-ras oncogene of Harvey rat sarcoma virus. Injection of this antibody into axolotl oocytes subjected to progesterone treatment does not prevent meiotic maturation. Injection of the same antibody into a blastomere of axolotl eggs at the 2- or 4-cell stage causes cleavage arrest in the descendants of the injected blastomere. Cytological observations of the injected eggs show, in the arrested blastomeres, enlarged nuclei always surrounded by an intact nuclear envelope and containing uncondensed chromatin. The possible role of ras protein in meiosis and mitosis is discussed.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.2.502