Allospecific Cytotoxic T Lymphocytes Recognize an H-2 Peptide in the Context of a Murine Major Histocompatibility Complex Class I Molecule

We have isolated cytotoxic T lymphocytes (CTL) preferentially reactive with the α 1 external domain of the H-2Ldantigen by selecting for T cells capable of recognizing a variant major histocompatibility complex (MHC) class I antigen sharing α 1 sequences with H-2Ld. Using these CTL, we demonstrate t...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-03, Vol.85 (6), p.1927-1931
Main Authors: Song, Elizabeth S., Linsk, Richard, Olson, Clifford A., McMillan, Minnie, Goodenow, Robert S.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analysis of the immune response. Humoral and cellular immunity
Animals
Antigens
Biological and medical sciences
Blasts
Cross Reactions
Fundamental and applied biological sciences. Psychology
Fundamental immunology
H-2 Antigens - immunology
Haploidy
Haplotypes
Histocompatibility Antigen H-2D
Immunobiology
Isoantigens - immunology
L cells
Major Histocompatibility Complex
Major histocompatibility complex genes
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Molecular Sequence Data
Molecular structure
Molecules
Organs and cells involved in the immune response
Reactivity
Spleen cells
T lymphocytes
T-Lymphocytes, Cytotoxic - immunology
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Summary:We have isolated cytotoxic T lymphocytes (CTL) preferentially reactive with the α 1 external domain of the H-2Ldantigen by selecting for T cells capable of recognizing a variant major histocompatibility complex (MHC) class I antigen sharing α 1 sequences with H-2Ld. Using these CTL, we demonstrate that a synthetic α 1 peptide corresponding to one of the helices derived from the H-2Ldmolecule can be presented by a class I restriction element to reconstitute a CTL determinant borne by intact H-2Ld. Moreover, several other H-2L-reactive CTL generated independently were also able to recognize H-2Ldeither as an intact alloantigen or as a peptide in conjunction with appropriate class I restriction elements. These data demonstrate that an H-2 peptide can reconstitute a CTL target structure and suggest that some alloreactive T cells in fact might be directed against allogeneic class I peptides in the context of a class I framework.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.6.1927