Interaction of Origin Binding Protein with an Origin of Replication of Herpes Simplex Virus 1

Herpes simplex virus 1 encodes a protein that binds specifically to a viral DNA replication origin (oriS). This origin-binding protein has been purified to homogeneity by means of sequence-specific DNA affinity chromatography. The purified origin-binding protein, which has a molecular weight of 83,0...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-05, Vol.85 (9), p.2959-2963
Main Authors: Elias, Per, Lehman, I. R.
Format: Article
Language:English
Subjects:
Base Sequence
Biochemistry
Biological and medical sciences
Cellulose nitrate
Chromatography
Chromatography, Affinity
DNA
DNA, Viral - biosynthesis
DNA-Binding Proteins - metabolism
Dyadic relations
Electrophoresis
Fundamental and applied biological sciences. Psychology
Gels
herpes simplex virus 1
Human herpesvirus 1
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Molecular Weight
Oligonucleotides
Repetitive Sequences, Nucleic Acid
Replication
Replication origin
Simplexvirus - physiology
Viral Proteins - metabolism
Virus Replication
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Description
Summary:Herpes simplex virus 1 encodes a protein that binds specifically to a viral DNA replication origin (oriS). This origin-binding protein has been purified to homogeneity by means of sequence-specific DNA affinity chromatography. The purified origin-binding protein, which has a molecular weight of 83,000, interacts with both parts of the oriS dyad; however, the affinity for the two sites differs by an order of magnitude. The sequence TTCGCA occurs as a nearly perfect direct repeat within the two sites and may constitute the recognition sequence for the origin-binding protein.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.9.2959