Z-DNA-Forming Sequences are Spontaneous Deletion Hot Spots

Z-DNA-forming sequences are shown to elicit a biological response in Escherichia coli. Plasmids containing sequences capable of adopting the Z conformation (GC and CA/GT) are shown to be hot spots for spontaneous deletions. All the deletions involve an even number of base pairs. The distribution of...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-10, Vol.86 (19), p.7465-7469
Main Authors: Freund, Anne-Marie, Bichara, Marc, Robert P. P. Fuchs
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical Sciences
Chromosome Deletion
DNA
DNA Transposable Elements
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
DNA, Superhelical - genetics
Electrophoresis, Gel, Two-Dimensional
Frameshift mutation
Fundamental and applied biological sciences. Psychology
Gels
Genetic mutation
High frequencies
Life Sciences
Molecular and cellular biology
Molecular genetics
Mutagenesis. Repair
Mutation
Nucleic Acid Conformation
Nucleic acids
Other
Phenotype
Phenotypes
Plasmids
Topology
Z form DNA
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Summary:Z-DNA-forming sequences are shown to elicit a biological response in Escherichia coli. Plasmids containing sequences capable of adopting the Z conformation (GC and CA/GT) are shown to be hot spots for spontaneous deletions. All the deletions involve an even number of base pairs. The distribution of the deletion events shows that the process ends when the Z-DNA-forming sequence has been reduced to a size no longer capable of adopting the Z conformation at natural superhelical density.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.19.7465