Estrogen-Responsive Element of the Human pS2 Gene is an Imperfectly Palindromic Sequence

Using chimeric recombinants transfected into HeLa cells and a transient expression assay, we demonstrate that the 5′-flanking region of the pS2 gene from position -428 to position -324 exhibits both constitutive and estrogen-inducible enhancer activity. The estrogen-inducible activity, but not the c...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-02, Vol.86 (4), p.1218-1222
Main Authors: Berry, Meera, Nunez, Anne-Marie, Chambon, Pierre
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
AUTORADIOGRAPHY
Base Sequence
BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHIMERAS
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
Enhancer Elements, Genetic
ENZYMES
ESTERASES
Estrogen receptors
ESTROGENS
Estrogens - pharmacology
GENE REGULATION
GENES
Genes - drug effects
Genetic vectors
HELA CELLS
HeLa Cells - metabolism
HORMONES
Humans
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MESSENGER-RNA
Molecular Sequence Data
MOSAICISM
MUTAGENESIS
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - genetics
NUCLEASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
Plasmids
Promoter regions
Proteins
RADIOISOTOPES
Reporter genes
RNA
STEROID HORMONES
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
Transcription, Genetic - drug effects
Transfection
Trefoil Factor-1
Tumor Suppressor Proteins
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Description
Summary:Using chimeric recombinants transfected into HeLa cells and a transient expression assay, we demonstrate that the 5′-flanking region of the pS2 gene from position -428 to position -324 exhibits both constitutive and estrogen-inducible enhancer activity. The estrogen-inducible activity, but not the constitutive activity, was inhibited by antiestrogens. ICI 164,384 behaved as a pure antagonist, whereas hydroxytamoxifen was a partial agonist-antagonist. The estrogen-responsive element of the pS2 gene has been narrowed down by site-directed deletion mutagenesis to a 13-base-pair (position -405 to position -393) imperfectly palindromic sequence, which in isolation can confer estrogen inducibility to the heterologous rabbit β -globin gene promoter. On the other hand, the sequences responsible for the constitutive enhancer activity are spread over the entire region.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.4.1218