Complete Nucleotide Sequence of a Molecular Clone of Woodchuck Hepatitis Virus that is Infectious in the Natural Host

Woodchuck hepatitis virus (WHV) DNA was cloned from viral particles obtained from the serum of a woodchuck with a naturally acquired infection. The complete nucleotide sequence of the virus genome was determined and found to be 3323 base pairs long. Transfection experiments demonstrated that the rec...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-03, Vol.86 (6), p.1846-1849
Main Authors: Girones, Rosina, Cote, Paul J., Hornbuckle, William E., Tennant, Bud C., Gerin, John L., Purcell, Robert H., Miller, Roger H.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA
DNA, Recombinant
Fundamental and applied biological sciences. Psychology
Genes. Genome
Genomes
Ground hogs
Hepadnaviridae
Hepatitis B virus
Hepatitis Viruses - genetics
Hepatitis Viruses - physiology
Hepatitis, Viral, Animal - microbiology
Liver
Marmota
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleotide sequences
Nucleotides
Sciuridae
Transfection
Virus Replication
Viruses
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Summary:Woodchuck hepatitis virus (WHV) DNA was cloned from viral particles obtained from the serum of a woodchuck with a naturally acquired infection. The complete nucleotide sequence of the virus genome was determined and found to be 3323 base pairs long. Transfection experiments demonstrated that the recombinant WHV DNA was infectious in each of 18 woodchucks tested and established a chronic carrier state in 1 of 13 neonates and 3 of 5 adult animals. WHV DNA from serum particles from the chronically infected neonate was cloned and the nucleotide sequence of three independent recombinants was compared directly with that of the input recombinant DNA. The consensus sequence of the three progeny genomes was identical to that of the parental DNA sequence. Therefore, transfection of woodchuck livers with recombinant WHV DNA induces active virus replication and gene expression and yields progeny genomes that are faithful copies of the input virus genome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.6.1846