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Targeted Inactivation of the Insulin Receptor Gene in Mouse 3T3-L1 Fibroblasts Via Homologous Recombination

To study the role of the insulin receptor in determining adipocyte differentiation of the mouse cell line 3T3-L1, we have introduced a mutation that inactivates the insulin receptor gene by homologous recombination. In two independent clones, inactivation of one allele of the insulin receptor gene w...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-06, Vol.88 (11), p.4708-4712
Main Authors: Accili, Domenico, Taylor, Simeon I.
Format: Article
Language:English
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Summary:To study the role of the insulin receptor in determining adipocyte differentiation of the mouse cell line 3T3-L1, we have introduced a mutation that inactivates the insulin receptor gene by homologous recombination. In two independent clones, inactivation of one allele of the insulin receptor gene was associated with a 50-70% reduction in the number of insulin receptors. In addition, both clones were markedly impaired in their ability to differentiate into adipocytes. The defect in adipocyte-specific differentiation was corrected by expression of transfected human insulin receptor cDNA. These data suggest that the insulin receptor may play an important role in promoting differentiation of 3T3-L1 cells into adipocytes in vitro.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.11.4708