G alpha 16, a G protein alpha subunit specifically expressed in hematopoietic cells

Signal-transduction pathways mediated by guanine nucleotide-binding regulatory proteins (G proteins) determine many of the responses of hematopoietic cells. A recently identified gene encoding a G protein alpha subunit, G alpha 16, is specifically expressed in human cells of the hematopoietic lineag...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-07, Vol.88 (13), p.5587-5591
Main Authors: T T Amatruda, 3rd, D A Steele, V Z Slepak, M I Simon
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA - genetics
Gene Expression
genes
GTP-Binding Proteins - classification
GTP-Binding Proteins - genetics
GTP-Binding Proteins - metabolism
Hematopoietic Stem Cells - metabolism
Humans
Molecular Sequence Data
Restriction Mapping
RNA, Messenger - genetics
Tumor Cells, Cultured
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Summary:Signal-transduction pathways mediated by guanine nucleotide-binding regulatory proteins (G proteins) determine many of the responses of hematopoietic cells. A recently identified gene encoding a G protein alpha subunit, G alpha 16, is specifically expressed in human cells of the hematopoietic lineage. The G alpha 16 cDNA encodes a protein with predicted Mr of 43,500, which resembles the G q class of alpha subunits and does not include a pertussis toxin ADP-ribosylation site. In comparison with other G protein alpha subunits, the G alpha 16 predicted protein has distinctive amino acid sequences in the amino terminus, the region A guanine nucleotide-binding domain, and in the carboxyl-terminal third of the protein. Cell lines of myelomonocytic and T-cell phenotype express the G alpha 16 gene, but no expression is detectable in two B-cell lines or in nonhematopoietic cell lines. G alpha 16 gene expression is down-regulated in HL-60 cells induced to differentiate to neutrophils with dimethyl sulfoxide. Antisera generated from synthetic peptides that correspond to two regions of G alpha 16 specifically react with a protein of 42- to 43-kDa in bacterial strains that overexpress G alpha 16 and in HL-60 membranes. This protein is decreased in membranes from dimethyl sulfoxide-differentiated HL-60 cells and is not detectable in COS cell membranes. The restricted expression of this gene suggests that G alpha 16 regulates cell-type-specific signal-transduction pathways, which are not inhibited by pertussis toxin.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.13.5587