A β Subunit Mutation Disrupting the Catalytic Function of Escherichia coli RNA Polymerase

The substitution of the evolutionarily conserved Glu-813 for lysine in the β subunit of RNA polymerase (RNAP) causes a partial loss of function in the assembled RNAP. In the presence of the four ribonucleoside triphosphates, the mutant RNAP displayed a decreased frequency of promoter clearance and d...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-07, Vol.88 (14), p.6018-6022
Main Authors: Lee, Jookyung, Kashlev, Mikhail, Borukhov, Sergei, Goldfarb, Alex
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Base Sequence
Biochemistry
Biological and medical sciences
Cloning, Molecular
Codon
DNA
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
Enzymes
Enzymes and enzyme inhibitors
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gels
Genes, Bacterial
Genetic mutation
Kinetics
Macromolecular Substances
Molecular Sequence Data
Mutagenesis
Nucleotides
Oligomers
Plasmids
Promoter Regions, Genetic
Restriction Mapping
Ribonucleosides
RNA
Transferases
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Summary:The substitution of the evolutionarily conserved Glu-813 for lysine in the β subunit of RNA polymerase (RNAP) causes a partial loss of function in the assembled RNAP. In the presence of the four ribonucleoside triphosphates, the mutant RNAP displayed a decreased frequency of promoter clearance and diminished elongation rate. Both defects could be compensated by raising the ribonucleoside triphosphate concentration. In the abortive initiation reaction limited by the incomplete set of ribonucleoside triphosphates, the mutant RNAP generated aberrant patterns of products indicative of their enhanced loss from the RNAP-promoter complex. A model is proposed, attributing the multiple effect of the mutation to the malfunctioning of the RNAP active center.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.14.6018