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Immobilization of DNA for Scanning Probe Microscopy
Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demons...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1992-11, Vol.89 (21), p.10129-10133 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| Citations: | Items that cite this one |
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| cited_by | cdi_FETCH-LOGICAL-c620t-83b1e96a7d93aabe9c8e9cb052751ea05f0bb88fb4612c06efd73803f93bd93b3 |
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| container_end_page | 10133 |
| container_issue | 21 |
| container_start_page | 10129 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 89 |
| creator | Allison, D. P. Bottomley, L. A. Thundat, T. Brown, G. M. Woychik, R. P. Schrick, J. J. Jacobson, K. Bruce Warmack, R. J. |
| description | Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demonstrate that the problem of molecular instability to forces exerted by the probe tip, inherent with scanning probe microscopes, can be prevented. |
| doi_str_mv | 10.1073/pnas.89.21.10129 |
| format | article |
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P. ; Bottomley, L. A. ; Thundat, T. ; Brown, G. M. ; Woychik, R. P. ; Schrick, J. J. ; Jacobson, K. Bruce ; Warmack, R. J.</creator><creatorcontrib>Allison, D. P. ; Bottomley, L. A. ; Thundat, T. ; Brown, G. M. ; Woychik, R. P. ; Schrick, J. J. ; Jacobson, K. Bruce ; Warmack, R. J.</creatorcontrib><description>Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demonstrate that the problem of molecular instability to forces exerted by the probe tip, inherent with scanning probe microscopes, can be prevented.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.21.10129</identifier><identifier>PMID: 1438201</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Acetates ; Analytical biochemistry: general aspects, technics, instrumentation ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Deoxyribonucleic acid ; DNA ; DNA probes ; DNA, Bacterial - ultrastructure ; DNA, Circular - ultrastructure ; Drug Stability ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Image contrast ; Imaging ; Indicators and Reagents ; Medical research ; Mica ; Microscopes ; Microscopy - methods ; Microscopy, Scanning Tunneling - methods ; Molecular probes ; Molecules ; Phosphorus Radioisotopes ; Plasmids</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-11, Vol.89 (21), p.10129-10133</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1993 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Nov 1, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c620t-83b1e96a7d93aabe9c8e9cb052751ea05f0bb88fb4612c06efd73803f93bd93b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/21.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2360562$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2360562$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4474783$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1438201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Allison, D. 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The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demonstrate that the problem of molecular instability to forces exerted by the probe tip, inherent with scanning probe microscopes, can be prevented.</description><subject>Acetates</subject><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA probes</subject><subject>DNA, Bacterial - ultrastructure</subject><subject>DNA, Circular - ultrastructure</subject><subject>Drug Stability</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Image contrast</subject><subject>Imaging</subject><subject>Indicators and Reagents</subject><subject>Medical research</subject><subject>Mica</subject><subject>Microscopes</subject><subject>Microscopy - methods</subject><subject>Microscopy, Scanning Tunneling - methods</subject><subject>Molecular probes</subject><subject>Molecules</subject><subject>Phosphorus Radioisotopes</subject><subject>Plasmids</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqFkc9vFCEUx4nR1G317kHjpDHGy6wPmGEg8dJUrU3qj0Q9E2ChspmBFWaM9a8v465r9aAHQsj3833v8b4IPcCwxNDR55ug8pKLJcHljYm4hRYYBK5ZI-A2WgCQruYNae6iw5zXACBaDgfoADeUE8ALRM-HIWrf-x9q9DFU0VUv351ULqbqo1Eh-HBZfUhR2-qtNylmEzdX99Adp_ps7-_uI_T59atPp2_qi_dn56cnF7VhBMaaU42tYKpbCaqUtsLwcjS0pGuxVdA60JpzpxuGiQFm3aqjHKgTVBeLpkfoxbbuZtKDXRkbxqR6uUl-UOlKRuXln0rwX-Rl_CZbIAIX-9OdPcWvk82jHHw2tu9VsHHKsqOUcMbZf0HMaEs73hbw-C9wHacUyg5kWSYpGMzVYAvN68rJuv3AGOQcmpxDk1xIguXP0Irl0c2P_jZsUyr6k52uslG9SyoYn_dY03RNx2nBnu2wucEv9UYj6aa-H-33saCP_40W4uGWWOcxpj1CKIOWEXoNB23AyQ</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>Allison, D. 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P.</au><au>Bottomley, L. A.</au><au>Thundat, T.</au><au>Brown, G. M.</au><au>Woychik, R. P.</au><au>Schrick, J. J.</au><au>Jacobson, K. Bruce</au><au>Warmack, R. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of DNA for Scanning Probe Microscopy</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>89</volume><issue>21</issue><spage>10129</spage><epage>10133</epage><pages>10129-10133</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demonstrate that the problem of molecular instability to forces exerted by the probe tip, inherent with scanning probe microscopes, can be prevented.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1438201</pmid><doi>10.1073/pnas.89.21.10129</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1992-11, Vol.89 (21), p.10129-10133 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_89_21_10129_fulltext |
| source | JSTOR Archival Journals and Primary Sources Collection; PubMed Central |
| subjects | Acetates Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences Deoxyribonucleic acid DNA DNA probes DNA, Bacterial - ultrastructure DNA, Circular - ultrastructure Drug Stability Escherichia coli Fundamental and applied biological sciences. Psychology Image contrast Imaging Indicators and Reagents Medical research Mica Microscopes Microscopy - methods Microscopy, Scanning Tunneling - methods Molecular probes Molecules Phosphorus Radioisotopes Plasmids |
| title | Immobilization of DNA for Scanning Probe Microscopy |
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