A family of beta 7 integrins on human mucosal lymphocytes

• Published in: Proceedings of the National Academy of Sciences - PNAS 1992-03, Vol.89 (5), p.1924-1928
• Main Authors: Parker, C M, Cepek, K L, Russell, G J, Shaw, S K, Posnett, D N, Schwarting, R, Brenner, M B
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antibodies, Monoclonal - immunology; Antigens, Neoplasm - chemistry; Antigens, Neoplasm - immunology; Antigens, Neoplasm - metabolism; Humans; Integrin beta Chains; Integrins - chemistry; Integrins - immunology; Integrins - metabolism; Intestinal Mucosa - cytology; Macromolecular Substances; Mice; Molecular Sequence Data; Molecular Weight; Protein Precursors - metabolism; T-Lymphocytes - chemistry; T-Lymphocytes - cytology; Transforming Growth Factor beta - metabolism
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.89.5.1924

The heterodimeric protein complex recognized by the human mucosal lymphocyte 1 (HML-1) monoclonal antibody is expressed on 95% of intraepithelial lymphocytes but on only 1-2% of peripheral blood lymphocytes [Cerf-Bensusson, N., Jarry, A., Brousse, N., Lisowska-Grospierre, B., Guy-Grand, D. & Griscelli, C. (1987) Eur. J. Immunol. 17, 1279-1285]. We purified the smaller HML-1 subunit (105 kDa under nonreducing conditions) from hairy-cell leukemia cells and determined the N-terminal amino acid sequence of this chain. The 17 residues determined were identical to the deduced amino acid sequence encoded by an integrin beta 7 cDNA clone [Yuan, Q., Jiang, W.-M., Krissansen, G.W. & Watson, J.D. (1990) Int. Immunol. 2, 1097-1108]. Biochemical analysis of the larger HML-1 subunit (175 kDa under nonreducing conditions) suggested that it was a distinct member of the cleaved group of integrin alpha chains, which we designated alpha E. The beta 7 chain also was associated with the integrin alpha 4 subunit, suggesting that the HML-1 antigen (alpha E beta 7) and alpha 4 beta 7 constitute a beta 7 integrin family on mucosal lymphocytes. Interestingly, regulation of the expression of the HML-1 antigen was reciprocal to that of lymphocyte function-associated molecule 1 in the presence of transforming growth factor beta 1. We suggest that these beta 7 integrins may play a specific role in mucosal localization or adhesion and that the expression of the HML-1 antigen might be regulated by transforming growth factor beta 1 produced at or near epithelial tissues.