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Three-Dimensional Structure of Theiler Virus

Theiler murine encephalomyelitis virus strains are categorized into two groups, a neurovirulent group that rapidly kills the host, and a demyelinating group that causes a generally nonlethal infection of motor neurons followed by a persistent infection of the white matter with demyelinating lesions...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1992-03, Vol.89 (6), p.2061-2065
Main Authors: Grant, Robert A., Filman, David J., Fujinami, Robert S., Icenogle, Joseph P., Hogle, James M.
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container_title Proceedings of the National Academy of Sciences - PNAS
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Filman, David J.
Fujinami, Robert S.
Icenogle, Joseph P.
Hogle, James M.
description Theiler murine encephalomyelitis virus strains are categorized into two groups, a neurovirulent group that rapidly kills the host, and a demyelinating group that causes a generally nonlethal infection of motor neurons followed by a persistent infection of the white matter with demyelinating lesions similar to those found in multiple sclerosis. The three-dimensional structure of the DA strain, a member of the demyelinating group, has been determined at 2.8 Å resolution. As in other picornaviruses, the icosahedral capsid is formed by the packing of wedge-shaped eight-stranded antiparallel β barrels. The surface of Theiler virus has large star-shaped plateaus at the fivefold axes and broad depressions spanning the twofold axes. Several unusual structural features are clustered near one edge of the depression. These include two finger-like loops projecting from the surface (one formed by residues 78-85 of VP1, and the other formed by residues 56-65 of VP3) and a third loop containing three cysteines (residues 87, 89, and 91 of VP3), which appear to be covalently modified. Most of the sequence differences between the demyelinating and neurovirulent groups that could play a role in determining pathogenesis map to the surface of the star-shaped plateau. The distribution of these sequence differences on the surface of the virion is consistent with models in which the differences in the pathogenesis of the two groups of Theiler viruses are the result of differences in immunological or receptor-mediated recognition processes.
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source Open Access: PubMed Central; JSTOR Archival Journals and Primary Sources Collection
subjects Animals
Biological and medical sciences
Capsid - ultrastructure
Capsid proteins
Cell Line
Cellular biology
Demyelinating diseases
Electrical phases
Electron density
Fundamental and applied biological sciences. Psychology
Maus Elberfeld virus - ultrastructure
Medical research
Mengovirus
Microbiology
Models, Molecular
Models, Structural
Morphology, structure, chemical composition, physicochemical properties
Nervous system
Pathogenesis
Picornaviridae
Picornaviridae - ultrastructure
Protein Conformation
Viral morphology
Virions
Virology
Viruses
X-Ray Diffraction - methods
title Three-Dimensional Structure of Theiler Virus
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