Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene

Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-04, Vol.89 (7), p.2624-2628
Main Authors: Knutzon, D.S. (Calgene Inc., Davis, CA), Thompson, G.A, Radke, S.E, Johnson, W.B, Knauf, V.C, Kridl, J.C
Format: Article
Language:English
Subjects:
ACEITE DE COLZA
ACIDE GRAS SATURE
ACIDOS GRASOS SATURADOS
Base Sequence
Biological and medical sciences
Biology
BIOSINTESIS
BIOSYNTHESE
Biosynthesis
Biotechnology
Brassica - genetics
Brassica - growth & development
BRASSICA CAMPESTRIS
BRASSICA NAPUS
Canola
Cloning, Molecular
DNA - genetics
Enzymes
EXPRESION GENICA
EXPRESSION DES GENES
Fatty acids
Flowers & plants
Fundamental and applied biological sciences. Psychology
Gene Expression
Genetic Engineering
Genetic technics
GENETICA MOLECULAR
Genetics
GENETIQUE MOLECULAIRE
GENIE GENETIQUE
HUILE DE COLZA
INGENIERIA GENETICA
Kanamycin - pharmacology
METABOLISME DES LIPIDES
METABOLISMO DE LIPIDOS
Methods. Procedures. Technologies
Mixed Function Oxygenases - genetics
Mixed Function Oxygenases - metabolism
Molecular Sequence Data
Oils & fats
Phenotypes
Plant Oils - metabolism
Plants
RNA, Antisense
RNA, Messenger - genetics
Seedlings
Stearates
Stearates - metabolism
Transgenic animals and transgenic plants
Transgenic plants
Unsaturated fatty acids
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Summary:Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.7.2624