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Recovery of Respiration Following the SOS Response of Escherichia coli Requires RecA-Mediated Induction of 2-Keto-4-Hydroxyglutarate Aldolase

Agents that damage DNA in Escherichia coli or interfere with its replication induce DNA repair and mutagenesis via the SOS response. This well-known activity is regulated by the RecA protein and the LexA repressor. Following repair or bypass of the DNA lesion, the cell returns to its resting state b...

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Published in:	Proceedings of the National Academy of Sciences - PNAS 1995-12, Vol.92 (25), p.11806-11809
Main Authors:	Cayrol, C., Petit, C., Raynaud, B., Capdevielle, J., J.-C. Guillemot, Defais, M.
Format:	Article
Language:	English
Subjects:	Bacteria Base Sequence DNA Dose-Response Relationship, Radiation Enzyme Induction Escherichia coli Escherichia coli - enzymology Escherichia coli - physiology Escherichia coli - radiation effects Gels Gene expression regulation Gene Expression Regulation, Bacterial Genes Genetic SOS response Genetics Inductive reasoning Irradiation Kanamycin Resistance Metabolism Models, Biological Molecular Sequence Data Oxo-Acid-Lyases - biosynthesis Oxygen Consumption Proteins Rec A Recombinases - metabolism Respiration SOS Response (Genetics) Ultraviolet Rays - adverse effects
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cited_by	cdi_FETCH-LOGICAL-c525t-afbd39dbd920d6b59bdee7257b9d0c82119d3db67e0bc1952854d1da2d293d0e3
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container_issue	25
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container_title	Proceedings of the National Academy of Sciences - PNAS
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creator	Cayrol, C. Petit, C. Raynaud, B. Capdevielle, J. J.-C. Guillemot Defais, M.
description	Agents that damage DNA in Escherichia coli or interfere with its replication induce DNA repair and mutagenesis via the SOS response. This well-known activity is regulated by the RecA protein and the LexA repressor. Following repair or bypass of the DNA lesion, the cell returns to its resting state by a largely unknown process. We found that 2-keto-4-hydroxyglutarate aldolase (4-hydroxy-2-oxoglutarate aldolase; EC 4.1.3.16) is necessary for the recovery of respiration and that it is regulated by the SOS response. This protein was induced by DNA-damaging agents. Induction required RecA activation. When the LexA regulon was repressed, activation of RecA was not sufficient for induction, indicating the requirement for an additional protein under LexA control. Finally, a mutant in the corresponding hga gene was UV sensitive. 2-Keto-4-hydroxyglutarate aldolase also plays a role in respiratory metabolic pathways, which suggests a mechanism for respiration resumption during the termination of the SOS response.
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Guillemot</creatorcontrib><creatorcontrib>Defais, M.</creatorcontrib><title>Recovery of Respiration Following the SOS Response of Escherichia coli Requires RecA-Mediated Induction of 2-Keto-4-Hydroxyglutarate Aldolase</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Agents that damage DNA in Escherichia coli or interfere with its replication induce DNA repair and mutagenesis via the SOS response. This well-known activity is regulated by the RecA protein and the LexA repressor. Following repair or bypass of the DNA lesion, the cell returns to its resting state by a largely unknown process. We found that 2-keto-4-hydroxyglutarate aldolase (4-hydroxy-2-oxoglutarate aldolase; EC 4.1.3.16) is necessary for the recovery of respiration and that it is regulated by the SOS response. This protein was induced by DNA-damaging agents. Induction required RecA activation. When the LexA regulon was repressed, activation of RecA was not sufficient for induction, indicating the requirement for an additional protein under LexA control. 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Guillemot</au><au>Defais, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recovery of Respiration Following the SOS Response of Escherichia coli Requires RecA-Mediated Induction of 2-Keto-4-Hydroxyglutarate Aldolase</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1995-12-05</date><risdate>1995</risdate><volume>92</volume><issue>25</issue><spage>11806</spage><epage>11809</epage><pages>11806-11809</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Agents that damage DNA in Escherichia coli or interfere with its replication induce DNA repair and mutagenesis via the SOS response. This well-known activity is regulated by the RecA protein and the LexA repressor. Following repair or bypass of the DNA lesion, the cell returns to its resting state by a largely unknown process. 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subjects	Bacteria Base Sequence DNA Dose-Response Relationship, Radiation Enzyme Induction Escherichia coli Escherichia coli - enzymology Escherichia coli - physiology Escherichia coli - radiation effects Gels Gene expression regulation Gene Expression Regulation, Bacterial Genes Genetic SOS response Genetics Inductive reasoning Irradiation Kanamycin Resistance Metabolism Models, Biological Molecular Sequence Data Oxo-Acid-Lyases - biosynthesis Oxygen Consumption Proteins Rec A Recombinases - metabolism Respiration SOS Response (Genetics) Ultraviolet Rays - adverse effects
title	Recovery of Respiration Following the SOS Response of Escherichia coli Requires RecA-Mediated Induction of 2-Keto-4-Hydroxyglutarate Aldolase
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