Complementation of the ionizing radiation sensitivity, DNA end binding, and V(D)J recombination defects of double-strand break repair mutants by the p86 Ku autoantigen

Two ionizing radiation-sensitive (IRs) and DNA double-strand break (DSB) mutants, sxi-3 and sxi-2, were shown to be severely deficient in a DNA end binding activity, similar to a previously described activity of the Ku autoantigen, correlating with the xrs (XRCC5) mutations. Cell fusions with xrs-6,...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1995-01, Vol.92 (3), p.890-894
Main Authors: Boubnov, N V, Hall, K T, Wills, Z, Lee, S E, He, D M, Benjamin, D M, Pulaski, C R, Band, H, Reeves, W, Hendrickson, E A
Format: Article
Language:English
Subjects:
Animals
Antigens, Nuclear
Biochemistry
Cell Fusion
Cell Line
CHO Cells
Cricetinae
Deoxyribonucleic acid
DNA
DNA - metabolism
DNA Damage
DNA Helicases
DNA Repair
DNA-Binding Proteins - physiology
Genetic Complementation Test
Humans
Ku Autoantigen
Mutation
Mutation - physiology
Nuclear Proteins - physiology
Radiation Tolerance - genetics
Recombination, Genetic - genetics
RNA, Messenger - biosynthesis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two ionizing radiation-sensitive (IRs) and DNA double-strand break (DSB) mutants, sxi-3 and sxi-2, were shown to be severely deficient in a DNA end binding activity, similar to a previously described activity of the Ku autoantigen, correlating with the xrs (XRCC5) mutations. Cell fusions with xrs-6, another IRs, DSB repair-deficient cell line, defined these sxi mutants in the XRCC5 group. sxi-3 cells have low expression levels of the p86Ku mRNA. Introduction of the Ku p86 gene, but not the p70 Ku gene, complemented the IRs, DNA end binding, and variable (diversity) joining [V(D)J] recombination signal and coding junction deficiencies of sxi-3. Thus, the p86 Ku gene product is essential for DSB repair and V(D)J recombination.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.92.3.890