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Fork-Like DNA Templates Support Bypass Replication of Lesions that Block DNA Synthesis on Single-Stranded Templates

DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on...

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Published in:	Proceedings of the National Academy of Sciences - PNAS 1996-11, Vol.93 (24), p.13766-13769
Main Authors:	Hoffmann, Jean-Sebastien, Pillaire, Marie-Jeanne, Lesca, Claire, Burnouf, Dominique, Robert P. P. Fuchs, Defais, Martine, Villani, Giuseppe
Format:	Article
Language:	English
Subjects:	2-Acetylaminofluorene Adducts Animals Antineoplastic Agents Base Sequence Biochemistry Biological Sciences Carcinogens Cell extracts CHO Cells Cisplatin Cricetinae Deoxyribonucleic acid DNA DNA Adducts DNA Damage DNA Replication DNA, Single-Stranded - chemistry DNA, Single-Stranded - isolation & purification DNA, Single-Stranded - metabolism Genetics Lesions Molecular Sequence Data Nucleic Acid Conformation Oligonucleotides Platinum Templates, Genetic
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cited_by	cdi_FETCH-LOGICAL-c523t-e010a109d58e3df939ff0a9cd4f985c5b3fd71679ca3f789cd207b18838e4b3d3
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creator	Hoffmann, Jean-Sebastien Pillaire, Marie-Jeanne Lesca, Claire Burnouf, Dominique Robert P. P. Fuchs Defais, Martine Villani, Giuseppe
description	DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on DNA damage processing by the cellular replication machinery was tested using eukaryotic cell extracts and model DNA substrates containing site-specific DNA adducts formed by the anticancer drug cisplatin or by the carcinogen N-2-acetylaminofluorene. Bypass of both lesions was observed only with fork-like substrates, whereas complete inhibition of DNA synthesis occurred on damaged single-stranded DNA substrates. These results suggest a role for additional accessory factors that permit DNA polymerases to bypass lesions when present in fork-like DNA.
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source	JSTOR Archival Journals and Primary Sources Collection; PubMed Central
subjects	2-Acetylaminofluorene Adducts Animals Antineoplastic Agents Base Sequence Biochemistry Biological Sciences Carcinogens Cell extracts CHO Cells Cisplatin Cricetinae Deoxyribonucleic acid DNA DNA Adducts DNA Damage DNA Replication DNA, Single-Stranded - chemistry DNA, Single-Stranded - isolation & purification DNA, Single-Stranded - metabolism Genetics Lesions Molecular Sequence Data Nucleic Acid Conformation Oligonucleotides Platinum Templates, Genetic
title	Fork-Like DNA Templates Support Bypass Replication of Lesions that Block DNA Synthesis on Single-Stranded Templates
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