Sterol Regulation of Acetyl Coenzyme A Carboxylase: A Mechanism for Coordinate Control of Cellular Lipid

Transcription from the housekeeping promoter for the acetyl coenzyme A carboxylase (ACC) gene, which encodes the rate-controlling enzyme of fatty acid biosynthesis, is shown to be regulated by cellular sterol levels through novel binding sites for the sterol-sensitive sterol regulatory element bindi...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-02, Vol.93 (3), p.1049-1053
Main Authors: Lopez, Jose M., Bennett, Mary K., Sanchez, Hugo B., Rosenfeld, John M., Osborne, Timothy F.
Format: Article
Language:English
Subjects:
Acetyl-CoA Carboxylase - biosynthesis
Acetyl-CoA Carboxylase - genetics
Animals
Base Sequence
Binding Sites
Biochemistry
CCAAT-Enhancer-Binding Proteins
Cell Line
Cellular biology
Cholesterol
Cholesterol - metabolism
Cholesterols
Cloning, Molecular
Deoxyribonuclease I
DNA Footprinting
DNA, Complementary
DNA-Binding Proteins - metabolism
Enzymes
Fatty acids
Fatty Acids - metabolism
Gene expression regulation
Gene Expression Regulation, Enzymologic
Helix-Loop-Helix Motifs
Hep G2 cells
Homeostasis
Humans
Kinetics
LDL receptors
Lipid Metabolism
Lipids
Messenger RNA
Metabolism
Molecular biology
Molecular Sequence Data
Nuclear Proteins - metabolism
Plasmids
Polymerase Chain Reaction
Promoter regions
Promoter Regions, Genetic
Rats
Recombinant Proteins - biosynthesis
Sterol Regulatory Element Binding Protein 1
Sterols
Sterols - biosynthesis
TATA Box
Transcription Factors - metabolism
Transcription, Genetic
Transfection
Tumor Cells, Cultured
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transcription from the housekeeping promoter for the acetyl coenzyme A carboxylase (ACC) gene, which encodes the rate-controlling enzyme of fatty acid biosynthesis, is shown to be regulated by cellular sterol levels through novel binding sites for the sterol-sensitive sterol regulatory element binding protein (SREBP)-1 transcription factor. The position of the SREBP sites relative to those for the ubiquitous auxiliary transcription factor Sp1 is reminiscent of that previously described for the sterol-regulated low density lipoprotein receptor promoter. The experiments provide molecular evidence that the metabolism of fatty acids and cholesterol, two different classes of essential cellular lipids, are coordinately regulated by cellular lipid levels.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.3.1049