Photorepair mutants of Arabidopsis

UV radiation induces two major DNA damage products, the cyclobutane pyrimidine dimer (CPD) and, at a lower frequency, the pyrimidine (6-4) pyrimidinone dimer (6-4 product). Although Escherichia coli and Saccharomyces cerevisiae produce a CPD-specific photolyase that eliminates only this class of dim...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1997-07, Vol.94 (14), p.7441-7445
Main Authors: Jiang, C.Z. (University of California, Davis, CA.), Yee, J, Mitchell, D.L, Britt, A.B
Format: Article
Language:English
Subjects:
ADN
Arabidopsis - genetics
ARABIDOPSIS THALIANA
Biological Sciences
Botany
Carbon-Carbon Lyases - metabolism
Cephalopelvic disproportion
COMPLEMENTATION
Crotalus atrox
CYCLOBUTANE PYRIMIDINE DIMERS
CYCLOBUTANE PYRIMIDINE PHOTOLYASE
Deoxyribodipyrimidine Photo-Lyase - genetics
Deoxyribodipyrimidine Photo-Lyase - metabolism
Dimers
DNA
DNA damage
DNA MODIFICATION
DNA REPAIR
Drosophila melanogaster
Escherichia coli
Flowers & plants
Genetic mutation
GENETICA
GENETICS
GENETIQUE
Genomes
Lesions
LIASAS
LYASE
LYASES
MUTACION
MUTANT
MUTANTES
MUTANTS
MUTATION
PHOTOREACTIVATION
Pyrimidine dimers
Pyrimidine Dimers - metabolism
PYRIMIDINE PYRIMIDINONE DIMERS
RADIACION ULTRAVIOLETA
RAYONNEMENT ULTRAVIOLET
Root growth
Saccharomyces cerevisiae
Seedlings
ULTRAVIOLET RADIATION
Ultraviolet Rays
Xenopus laevis
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Summary:UV radiation induces two major DNA damage products, the cyclobutane pyrimidine dimer (CPD) and, at a lower frequency, the pyrimidine (6-4) pyrimidinone dimer (6-4 product). Although Escherichia coli and Saccharomyces cerevisiae produce a CPD-specific photolyase that eliminates only this class of dimer, Arabidopsis thaliana, Drosophila melanogaster, Crotalus atrox, and Xenopus laevis have recently been shown to photoreactivate both CPDs and 6-4 products. We describe the isolation and characterization of two new classes of mutants of Arabidopsis, termed uvr2 and uvr3, that are defective in the photoreactivation of CPDs and 6-4 products, respectively. We demonstrate that the CPD photolyase mutation is genetically linked to a DNA sequence encoding a type II (metazoan) CPD photolyase. In addition, we are able to generate plants in which only CPDs or 6-4 products are photoreactivated in the nuclear genome by exposing these mutants to UV light and then allowing them to repair one or the other class of dimers. This provides us with a unique opportunity to study the biological consequences of each of these two major UV-induced photoproducts in an intact living system
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.94.14.7441