Dictyostelium myosin II null mutant can still cap Con A receptors

Cross-linked antigens on the surface of a motile cell cap at the trailing end of the cell. In Dictyostelium discoideum, myosin II null mutants have previously been reported to be unable to cap Con A receptors, although they are able to locomote. This finding implicated myosin II as an essential comp...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1997-09, Vol.94 (18), p.9684-9686
Main Authors: Aguado-Velasco, C, Bretscher, M.S
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
BIOCHEMISTRY
BIOCHIMIE
Biological Sciences
BIOQUIMICA
CAPPING
Cells
Cellular biology
CHEMICAL REACTIONS
CONCANAVALIN A
CONCANAVALINE A
CROSS LINKING
CYTOCHEMISTRY
Cytoskeleton
DICTYOSTELIUM
Dictyostelium - genetics
Dictyostelium - metabolism
DICTYOSTELIUM DISCOIDEUM
Fluorescence
GLOBULINAS
GLOBULINE
GLOBULINS
Histograms
Locomotion
Membrane proteins
MUTANT
MUTANTES
MUTANTS
Mutation
Myosins - genetics
Myosins - metabolism
REACCIONES QUIMICAS
REACTION CHIMIQUE
Receptors
Receptors, Concanavalin A - metabolism
Surface antigens
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Description
Summary:Cross-linked antigens on the surface of a motile cell cap at the trailing end of the cell. In Dictyostelium discoideum, myosin II null mutants have previously been reported to be unable to cap Con A receptors, although they are able to locomote. This finding implicated myosin II as an essential component of the capping mechanism, although not of the machinery for locomotion. Here we show that myosin II null mutants do cap Con A receptors, albeit less efficiently than does wild type. This shows that cap formation is not absolutely dependent on myosin II and that a close mechanistic relationship between capping, particle movement, and cell migration may still exist.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.94.18.9684