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Dictyostelium myosin II null mutant can still cap Con A receptors

Cross-linked antigens on the surface of a motile cell cap at the trailing end of the cell. In Dictyostelium discoideum, myosin II null mutants have previously been reported to be unable to cap Con A receptors, although they are able to locomote. This finding implicated myosin II as an essential comp...

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Published in:	Proceedings of the National Academy of Sciences - PNAS 1997-09, Vol.94 (18), p.9684-9686
Main Authors:	Aguado-Velasco, C, Bretscher, M.S
Format:	Article
Language:	English
Subjects:	Animals Antibodies Antigens BIOCHEMISTRY BIOCHIMIE Biological Sciences BIOQUIMICA CAPPING Cells Cellular biology CHEMICAL REACTIONS CONCANAVALIN A CONCANAVALINE A CROSS LINKING CYTOCHEMISTRY Cytoskeleton DICTYOSTELIUM Dictyostelium - genetics Dictyostelium - metabolism DICTYOSTELIUM DISCOIDEUM Fluorescence GLOBULINAS GLOBULINE GLOBULINS Histograms Locomotion Membrane proteins MUTANT MUTANTES MUTANTS Mutation Myosins - genetics Myosins - metabolism REACCIONES QUIMICAS REACTION CHIMIQUE Receptors Receptors, Concanavalin A - metabolism Surface antigens
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creator	Aguado-Velasco, C Bretscher, M.S
description	Cross-linked antigens on the surface of a motile cell cap at the trailing end of the cell. In Dictyostelium discoideum, myosin II null mutants have previously been reported to be unable to cap Con A receptors, although they are able to locomote. This finding implicated myosin II as an essential component of the capping mechanism, although not of the machinery for locomotion. Here we show that myosin II null mutants do cap Con A receptors, albeit less efficiently than does wild type. This shows that cap formation is not absolutely dependent on myosin II and that a close mechanistic relationship between capping, particle movement, and cell migration may still exist.
doi_str_mv	10.1073/pnas.94.18.9684
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This shows that cap formation is not absolutely dependent on myosin II and that a close mechanistic relationship between capping, particle movement, and cell migration may still exist.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.94.18.9684</identifier><identifier>PMID: 9275184</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies ; Antigens ; BIOCHEMISTRY ; BIOCHIMIE ; Biological Sciences ; BIOQUIMICA ; CAPPING ; Cells ; Cellular biology ; CHEMICAL REACTIONS ; CONCANAVALIN A ; CONCANAVALINE A ; CROSS LINKING ; CYTOCHEMISTRY ; Cytoskeleton ; DICTYOSTELIUM ; Dictyostelium - genetics ; Dictyostelium - metabolism ; DICTYOSTELIUM DISCOIDEUM ; Fluorescence ; GLOBULINAS ; GLOBULINE ; GLOBULINS ; Histograms ; Locomotion ; Membrane proteins ; MUTANT ; MUTANTES ; MUTANTS ; Mutation ; Myosins - genetics ; Myosins - metabolism ; REACCIONES QUIMICAS ; REACTION CHIMIQUE ; Receptors ; Receptors, Concanavalin A - metabolism ; Surface antigens</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1997-09, Vol.94 (18), p.9684-9686</ispartof><rights>Copyright 1993-1997 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Sep 2, 1997</rights><rights>Copyright © 1997, The National Academy of Sciences of the USA 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-83b829d3615685ca72d5da1003326c3d93657ce0cbc0b6e6a6a9fb05f61abeca3</citedby><cites>FETCH-LOGICAL-c540t-83b829d3615685ca72d5da1003326c3d93657ce0cbc0b6e6a6a9fb05f61abeca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/94/18.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/43085$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/43085$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792,58237,58470</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9275184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aguado-Velasco, C</creatorcontrib><creatorcontrib>Bretscher, M.S</creatorcontrib><title>Dictyostelium myosin II null mutant can still cap Con A receptors</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Cross-linked antigens on the surface of a motile cell cap at the trailing end of the cell. 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subjects	Animals Antibodies Antigens BIOCHEMISTRY BIOCHIMIE Biological Sciences BIOQUIMICA CAPPING Cells Cellular biology CHEMICAL REACTIONS CONCANAVALIN A CONCANAVALINE A CROSS LINKING CYTOCHEMISTRY Cytoskeleton DICTYOSTELIUM Dictyostelium - genetics Dictyostelium - metabolism DICTYOSTELIUM DISCOIDEUM Fluorescence GLOBULINAS GLOBULINE GLOBULINS Histograms Locomotion Membrane proteins MUTANT MUTANTES MUTANTS Mutation Myosins - genetics Myosins - metabolism REACCIONES QUIMICAS REACTION CHIMIQUE Receptors Receptors, Concanavalin A - metabolism Surface antigens
title	Dictyostelium myosin II null mutant can still cap Con A receptors
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