An Arabidopsis photolyase mutant is hypersensitive to ultraviolet-B radiation

Photolyases are DNA repair enzymes that use energy from blue light to repair pyrimidine dimers. We report the isolation of an Arabidopsis thaliana mutant (uvr2-1) that is defective in photorepair of cyclobutylpyrimidine dimers (CPDs). Whereas uvr2-1 is indistinguishable from wild type in the absence...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1997-01, Vol.94 (1), p.328-332
Main Authors: Landry, L.G. (Monmouth University, West Long Branch, NJ.), Stapleton, A.E, Lim, J, Hoffman, P, Hays, J.B, Walbot, V, Last, R.L
Format: Article
Language:English
Subjects:
ADN
Apoenzymes - genetics
Arabidopsis
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis - radiation effects
ARABIDOPSIS THALIANA
Biological Sciences
Botany
CARTE GENETIQUE
Cephalopelvic disproportion
Deoxyribodipyrimidine Photo-Lyase - genetics
Dimers
DNA damage
DNA Repair - genetics
Dose-Response Relationship, Radiation
ENZIMAS
ENZYME
Enzymes
FEUILLE
Fungal Proteins
GENE
GENE RECESSIF
GENES
GENES RECESIVOS
Genetic mutation
HOJAS
Lamps
Light
LUMIERE
LUZ
MAPAS GENETICOS
Membrane Glycoproteins
MUTACION
Mutagenesis
MUTANT
MUTANTES
MUTATION
Plant cells
Plants
Pyrimidine Dimers - metabolism
RACINE
RADIACION ULTRAVIOLETA
Radiation Tolerance - genetics
RAICES
RAYONNEMENT ULTRAVIOLET
Seedlings
Ultraviolet radiation
Ultraviolet Rays - adverse effects
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Summary:Photolyases are DNA repair enzymes that use energy from blue light to repair pyrimidine dimers. We report the isolation of an Arabidopsis thaliana mutant (uvr2-1) that is defective in photorepair of cyclobutylpyrimidine dimers (CPDs). Whereas uvr2-1 is indistinguishable from wild type in the absence of UV light, low UV-B levels inhibit growth and cause leaf necrosis. uvr2-1 is more sensitive to UV-B than wild type when placed under white light after UV-B treatment. In contrast, recovery in darkness or in light lacking photoreactivating blue light results in equal injury in uvr2-1 and wild type. The uvr2-1 mutant is unable to remove CPDs in vivo, and plant extracts lack detectable photolyase activity. This recessive mutation segregates as a single gene located near the top of chromosome 1, and is a structural gene mutation in the type II CPD photolyase PHR1. This mutant provides evidence that CPD photolyase is required for plant survival in the presence of UV-B light
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.94.1.328