Selection of marker-free transgenic plants using the isopentenyl transferase gene

We have developed a new plant vector system for repeated transformation (called MAT for multi-autotransformation) in which a chimeric ipt gene, inserted into the transposable element Ac, is used as a selectable marker for transformation. Selectable marker genes conferring antibiotic or herbicide res...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1997-03, Vol.94 (6), p.2117-2121
Main Authors: Ebinuma, H. (Nippon Paper Industries Co., Ltd., Tokyo, Japan.), Sugita, K, Matsunaga, E, Yamakado, M
Format: Article
Language:English
Subjects:
ADN RECOMBINADO
ADN RECOMBINE
Adventitious shoots
AGROBACTERIUM TUMEFACIENS
Biological Sciences
CULTIVO DE TEJIDOS
CULTURE DE TISSU
Cytokinins
Flowers & plants
GENE
GENES
Genetic engineering
Genetic transposition
Genetic vectors
HIBRIDOS
HYBRIDE
Hybridity
MARCADORES GENETICOS
MARQUEUR GENETIQUE
NICOTIANA TABACUM
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants
Plasmids
POPULUS
POPULUS GRANDIDENTATA
Populus sieboldii
REGENERACION IN VITRO
REGENERATION IN VITRO
Row transformations
SELECCION IN VITRO
SELECTION IN VITRO
TALLO
TIGE
Tobacco
TRANSFERASAS
TRANSFERASE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transgenic plants
Trees
ZEA MAYS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed a new plant vector system for repeated transformation (called MAT for multi-autotransformation) in which a chimeric ipt gene, inserted into the transposable element Ac, is used as a selectable marker for transformation. Selectable marker genes conferring antibiotic or herbicide resistance, used to introduce economically valuable genes into crop plants, have three major problems: (i) the selective agents have negative effects on proliferation and differentiation of plant cells; (ii) there is uncertainty regarding the environmental impact of many selectable marker genes; (iii) it is difficult to perform recurrent transformations using the same selectable marker to pyramid desirable genes. The MAT vector system containing the ipt gene and the Ac element is designed to overcome these difficulties. When tobacco leaf segments were transformed and selected, subsequent excision of the modified Ac produced marker-free transgenic tobacco plants without sexual crosses or seed production. In addition, the chimeric ipt gene could be visually used as a selectable marker for transformation of hybrid aspen (Populus sieboldii x Populus grandidentata). The chimeric ipt gene, therefore, is an attractive alternative to the most widely used selectable marker genes. The MAT vector system provides a promising way to shorten breeding time for genetically engineered crops. This method could be particularly valuable for fruit and forest trees, for which long generation times are a more significant barrier to breeding and genetic analysis
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.94.6.2117