Self and Viral Peptides can Initiate Lysis by Autologous Natural Killer Cells

Natural killer (NK) cells are inhibited by specific allotypes of class I major histocompatibility complex ligands recognized by polymorphic inhibitory receptors (e.g., NKIR1 and NKIR2). NK1- and NK2-specific clones recognize two groups of HLA-C allotypes that are distinguished by a dimorphism at res...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1997-04, Vol.94 (9), p.4604-4609
Main Authors: Mandelboim, Ofer, Wilson, S. Brian, Vales-Gomez, Mar, Reyburn, Hugh T., Strominger, Jack L.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biological Sciences
Cell lines
Cellular biology
Computer Simulation
Cytotoxicity, Immunologic
Diabetes
Enterovirus - immunology
Epitopes
Epitopes - immunology
Gene Products, tax - immunology
Glutamate Decarboxylase - immunology
HLA C antigens
HLA-C Antigens - immunology
Human T-lymphotropic virus 1 - immunology
Humans
Immunity (Disease)
Killer Cells, Natural - immunology
Models, Molecular
Molecular Sequence Data
Molecules
Natural killer cells
Natural killer T cells
Peptide Fragments - immunology
Peptides
Receptors
Sequence Alignment
T lymphocytes
Viral Proteins - immunology
Viruses
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Summary:Natural killer (NK) cells are inhibited by specific allotypes of class I major histocompatibility complex ligands recognized by polymorphic inhibitory receptors (e.g., NKIR1 and NKIR2). NK1- and NK2-specific clones recognize two groups of HLA-C allotypes that are distinguished by a dimorphism at residue 80 in the α 1 helix (α Lys-80 and α Asn-80, respectively). ``Empty'' HLA-Cw7 expressed in peptide transporter-deficient cells and HLA-Cw7 loaded with several peptides each functioned as inhibitory ligands for NK2 lines and clones. However, loading of HLA-Cw7 with two other peptides derived from glutamic acid decarboxylase or coxsackie virus (each of which has been associated with autoimmune diabetes mellitus) abrogated this inhibitory recognition. Both peptides contained Lys at P8 of the epitope. Substitution of P8 with Ala or two other basic amino acids, His and Arg, resulted in peptides that were inhibitory, as were peptides with P8 Val, Glu, or Asn. The manner in which a Lys at P8 might affect recognition is discussed, together with a hypothesis for a novel mechanism by which an autoimmune disease might be initiated.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.94.9.4604