Genes Controlling Fertilization-Independent Seed Development in Arabidopsis thaliana

We have cloned two genes, FIS1 and FIS2, that control both fertilization independent seed development and postpollination embryo development in Arabidopsis. These genes confer female gametophytic phenotypes. FIS2 encodes a protein with a C2H2zinc-finger motif and three putative nuclear localization...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1999-01, Vol.96 (1), p.296-301
Main Authors: Luo, Ming, Bilodeau, Pierre, Koltunow, Anna, Dennis, Elizabeth S., Peacock, W. James, Chaudhury, Abdul M.
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Arabidopsis
Arabidopsis - genetics
Arabidopsis Proteins
Biological Sciences
Chromosome Mapping
Complementary DNA
Cosmids
DNA
DNA, Complementary - genetics
Drosophila Proteins
Endosperm
Ethylenes
Genes
Genes, Plant
Genetic Complementation Test
Genetic mutation
Molecular Sequence Data
Nuclear Proteins - genetics
Plant Proteins - genetics
Plants
Polycomb Repressive Complex 2
Proteins
Repressor Proteins - genetics
Seed development
Seeds - growth & development
Sequence Homology, Amino Acid
Transcription Factors - genetics
Zinc Fingers - genetics
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Summary:We have cloned two genes, FIS1 and FIS2, that control both fertilization independent seed development and postpollination embryo development in Arabidopsis. These genes confer female gametophytic phenotypes. FIS2 encodes a protein with a C2H2zinc-finger motif and three putative nuclear localization signals, indicating that it is likely to be a transcription factor. FIS1 encodes a protein with homology to the Drosophila Polycomb group gene Enhancer-of-zeste and is identical to the recently described Arabidopsis gene MEDEA. FIS1 is a protein with a number of putative functional domains, including the SET domain present in Enhancer-of-zeste-related proteins. Comparison of the position of the lesions in the fis1 and medea mutant alleles indicates that fis1 is a null allele producing a truncated polypeptide lacking all the protein domains whereas the deduced protein from medea lacks only the SET domain. We present a model of the role of FIS1 and FIS2 gene products in seed development.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.96.1.296