Expression of Guanylin in "Pars Tuberalis-Specific Cells" and Gonadotrophs of Rat Adenohypophysis

The intestinal peptide guanylin regulates the electrolyte/water transport in the gastrointestinal epithelium by paracrine/luminocrine mechanisms. Because guanylin also circulates in the blood, we investigated the rat hypothalamo-pituitary region for expression and cellular localization of this pepti...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-02, Vol.97 (3), p.1131-1136
Main Authors: D'Este, Loredana, Kulaksiz, Hasan, Rausch, Ulrich, Vaccaro, Rosa, Wenger, Tibor, Tokunaga, Yoshimitsu, Renda, Tindaro G., Cetin, Yalcin
Format: Article
Language:English
Subjects:
adenohypophysis
Animals
Anterior pituitary
Biological Sciences
Blood vessels
Cells
Cellular biology
Complementary DNA
Duodenum
Endocrine cells
Epithelial cells
Female
Gastrointestinal Hormones
Gels
guanylin
hypothalamo-hypophyseal system
Hypothalamo-Hypophyseal System - physiology
Male
Microscopy, Immunoelectron
Natriuretic Peptides
Peptides
Peptides - genetics
Peptides - metabolism
Pituitary gland
Pituitary Gland, Anterior - metabolism
Pituitary Gland, Anterior - ultrastructure
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Rodents
Subcellular Fractions - metabolism
Water-Electrolyte Balance - physiology
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Summary:The intestinal peptide guanylin regulates the electrolyte/water transport in the gastrointestinal epithelium by paracrine/luminocrine mechanisms. Because guanylin also circulates in the blood, we investigated the rat hypothalamo-pituitary region for expression and cellular localization of this peptide. Reverse transcriptase-PCR analyses with guanylin-specific primers revealed expression of the peptide in the pars tuberalis and pars distalis of the pituitary. Western blotting analyses in hypophyseal tissue extracts identified the expected 12.5-kDa immunoreactive peptide by using two different region-specific guanylin antisera. Light and electron microscopic immunocytochemistry with the same antisera localized guanylin in "pars tuberalis-specific cells" in the juxtaneural pars tuberalis adjacent to nerve endings and blood vessels of the hypothalamo-pituitary portal system and in gonadotrophic cells within the distal pars tuberalis and ventrolateral part of the pars distalis. The presence and cell-specific localization of guanylin within the hypothalamo-hypophyseal system indicate that this peptide may be specifically involved in paracrine and endocrine regulatory mechanisms.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.97.3.1131